Osmotic and lonic Regulation in Chara L-cell Fragments
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概要
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Ion absorption from rather complicated artificial pond water (APW) by cell fragments having a lower osmotic pressure than the intact internodal cell (L-cell fragments) was studied. L-cell fragments were prepared by taking advantage of transcellular osmosis and ligating the cell with thread. The results were as follows: (1) L-cell fragments absorbed more K^+ than Na^+ from a KCL+NaCl mixture in the presence of Ca^<2+>. Mg^<2+> and SO_4^<2-> in the light; (2) the influx of KCl was larger than that of KNO_3; (3) the amount of positive charge carried by K^+, Na^+ and Mg^<2+> across the cell membrane balanced well with the amount of negative charge carried by Cl^- in Cl^--containing and NO_3^--free APW; (4) no conclusion could be made as to whether or not the rule of electroneutrality held for the K^+, Na^+, Ca^<2+> and NO_3^- fluxes across the cell membrane, because diluted KNO_3 is unstable; (5) L-cell fragments in KCl-containing APW survived longer than those in KNO_3-containing and Cl^--free APW; (6) after incubation in KNO_3-containing and Cl^--free APW, L-cell fragments absorbed a great amount of KCl immediately after being transferred to KCl-containing and NO_3^--free APW; and (7) lowering the turgor pressure of the intact cell by raising the external osmotic pressure did not induce ion flux into the cell. Thus, we concluded that the L-cell fragments absorbed ions from the external solution not because of their lower turgor pressure, but because of the diluted ion concentration of the cytoplasm and the vacuole. The electroneutrality rule held, at least, for K^+, Na^+, Mg^<2+> and Cl^- influxes across the cell membrane in the KCl-containing and NO_3^--free APW. These results were analyzed on the basis of an extended pore model which presumed the existence of ATP-dependent processes in the membrane, and suggested that K^+, Na^+ and Mg^<2+> inflows into an. L-cell fragment are likely to be induced by active Cl^- inflow.
- 日本植物生理学会の論文
著者
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OKIHARA Kiyoshi
Department of Applied Physiology, National Institute of Agrobiological Resources
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Okihara Kiyoshi
Department Of Applied Physiology National Institute Of Agrobiological Resources
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Okihara Kiyoshi
Department Of Biophysical Engineering Faculty Of Engineering Science Osaka University
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Kiyosawa Keitaro
Department of Biophysical Engineering. Faculty of Engineering Science, Osaka University
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Kiyosawa Keitaro
Department Of Biophysical Engineering Faculty Of Engineering Science Osaka University
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