Identification of the glutathione S-transferase gene responsible for flower color intensity in carnations
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概要
- 論文の詳細を見る
Two cDNAs with homology to glutathione S-transferase (GST) were isolated from the carnation (Dianthus caryophyllus); these cDNAs are termed here DcGSTF1 and DcGSTF2. Phylogenetic analysis suggested that both DcGSTF1 and DcGSTF2 belonged to the Phi class of GSTs. DcGSTF2 showed high levels of transcription at late stages of petal development when anthocyanin biosynthesis is most active. Sequencing of DcGSTF2 indicated that it consisted of three exons and two introns. A truncated DcGSTF2 gene, resulting from the insertion of a CACTA-type transposable element, was found in the genome of a mutable flower line bearing deep pink sectors on pale pink petals. A full length DcGSTF2 gene driven by a continuous expression promoter was introduced into the epidermal cells of carnations with pale pink petals. The transformed cells were deep pink. These results suggest that the DcGSTF2 gene is responsible for flower color intensity in carnations.
- 日本植物細胞分子生物学会の論文
- 2012-06-25
著者
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SASAKI Nobuhiro
Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agricultur
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Umemoto Naoyuki
Central Lab. Key Tech. Kirin Brewery Co.
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Uchida Yasuhiro
Department Of Cardiology Kainan Hospital
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Ito Y
National Inst. Agrobiological Sci. Ibaraki Jpn
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Ozeki Yoshihiro
Dept. Biotech. Fac. Tech. Tokyo Univ. Agri. & Tech.
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Ozeki Yoshihiro
Department Of Biology College Of Arts And Science The University Of Tokyo
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Ito Yoko
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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YOSHIDA Hiroyuki
JT・APRL
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YAMAGUCHI Masaatsu
S-Kyushu Univ.
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YOSHIDA Hiroyuki
Applied Plant Research Laboratory, Japan Tobacco, Inc.
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Ozeki Y
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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Sasaki Nobuhiro
Dep. Of Biotechnology Tokyo Univ. Of Agriculture And Technol.
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Itoh Yoshio
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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Sasaki Nobuhiro
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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NAKAYAMA Masayoshi
Institute of Floricultural Science, National Agriculture and Food Research Organization
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Okamura Masachika
Central Laboratories For Frontier Technology Kirin Holdings Co. Ltd.
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NISHIZAKI Yuzo
Department of Biotechnology, Tokyo University of Agriculture and Technology
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WAKAMATSU Eigo
Department of Biotechnology, Tokyo University of Agriculture and Technology
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MOMOSE Masaki
Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd.
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YAMAGUCHI Masaatsu
Faculty of Enviromental Horticulture, Minami Kyushu University
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Yamaguchi Masaatsu
Faculty Of Enviromental Horticulture Minami Kyushu University
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Wakamatsu Eigo
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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Nishizaki Yuzo
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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Nakayama Masayoshi
Institute Of Floricultural Science National Agriculture And Food Research Organization
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Uchida Yasuhiro
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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Momose Masaki
Central Laboratories For Frontier Technology Kirin Holdings Co. Ltd.
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Yoshida Hiroyuki
Applied Plant Research Laboratory Japan Tobacco Inc.
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UMEMOTO Naoyuki
Central Lab. Key Tech. , Kirin Brewery Co.
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Itoh Yoshio
Department of Applied Chemistry, Faculty of Engineering, Tohoku University
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