Identification of the glutathione S-transferase gene responsible for flower color intensity in carnations

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概要

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• Two cDNAs with homology to glutathione S-transferase (GST) were isolated from the carnation (Dianthus caryophyllus); these cDNAs are termed here DcGSTF1 and DcGSTF2. Phylogenetic analysis suggested that both DcGSTF1 and DcGSTF2 belonged to the Phi class of GSTs. DcGSTF2 showed high levels of transcription at late stages of petal development when anthocyanin biosynthesis is most active. Sequencing of DcGSTF2 indicated that it consisted of three exons and two introns. A truncated DcGSTF2 gene, resulting from the insertion of a CACTA-type transposable element, was found in the genome of a mutable flower line bearing deep pink sectors on pale pink petals. A full length DcGSTF2 gene driven by a continuous expression promoter was introduced into the epidermal cells of carnations with pale pink petals. The transformed cells were deep pink. These results suggest that the DcGSTF2 gene is responsible for flower color intensity in carnations.

• 日本植物細胞分子生物学会の論文
• 2012-06-25

著者

• SASAKI Nobuhiro

  Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agricultur

• Umemoto Naoyuki

  Central Lab. Key Tech. Kirin Brewery Co.

• Uchida Yasuhiro

  Department Of Cardiology Kainan Hospital

• Ito Y

  National Inst. Agrobiological Sci. Ibaraki Jpn

• Ozeki Yoshihiro

  Dept. Biotech. Fac. Tech. Tokyo Univ. Agri. & Tech.

• Ozeki Yoshihiro

  Department Of Biology College Of Arts And Science The University Of Tokyo

• Ito Yoko

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• YOSHIDA Hiroyuki

  JT・APRL

• YAMAGUCHI Masaatsu

  S-Kyushu Univ.

• YOSHIDA Hiroyuki

  Applied Plant Research Laboratory, Japan Tobacco, Inc.

• Ozeki Y

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• Sasaki Nobuhiro

  Dep. Of Biotechnology Tokyo Univ. Of Agriculture And Technol.

• Itoh Yoshio

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• Sasaki Nobuhiro

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• NAKAYAMA Masayoshi

  Institute of Floricultural Science, National Agriculture and Food Research Organization

• Okamura Masachika

  Central Laboratories For Frontier Technology Kirin Holdings Co. Ltd.

• NISHIZAKI Yuzo

  Department of Biotechnology, Tokyo University of Agriculture and Technology

• WAKAMATSU Eigo

  Department of Biotechnology, Tokyo University of Agriculture and Technology

• MOMOSE Masaki

  Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd.

• YAMAGUCHI Masaatsu

  Faculty of Enviromental Horticulture, Minami Kyushu University

• Yamaguchi Masaatsu

  Faculty Of Enviromental Horticulture Minami Kyushu University

• Wakamatsu Eigo

  Department Of Biotechnology Tokyo University Of Agriculture And Technology

• Nishizaki Yuzo

  Department Of Biotechnology Tokyo University Of Agriculture And Technology

• Nakayama Masayoshi

  Institute Of Floricultural Science National Agriculture And Food Research Organization

• Uchida Yasuhiro

  Department Of Biotechnology Tokyo University Of Agriculture And Technology

• Momose Masaki

  Central Laboratories For Frontier Technology Kirin Holdings Co. Ltd.

• Yoshida Hiroyuki

  Applied Plant Research Laboratory Japan Tobacco Inc.

• UMEMOTO Naoyuki

  Central Lab. Key Tech. , Kirin Brewery Co.

• Itoh Yoshio

  Department of Applied Chemistry, Faculty of Engineering, Tohoku University

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