Protein profile of silkworm midgut of fifth-instar day-3 larvae
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概要
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Proteomic analysis was performed on the midgut of fifth-instar day-3 female silkworm (Bombyx mori, strain p50) larvae. Though silkworm genome analysis has not yet been completed, the Drosophila genome and silkworm expression sequence tag (EST) data were applied to the analysis of the midgut proteins in the database (DB) for identification. The spots, which were excised manually from two-dimensional electrophoresis (2-DE) gels, were treated with 4-vinylpyridine for alkylation. Each spot was analyzed by capillary high-performance liquid chromatography coupled with ion-trap mass spectrometry (MS) after proteolysis using a trypsin. Nearly 60% of the proteins analyzed by MS were identified. These included many kinds of cytoskeleton proteins, ATPase, and chaperonins.
- 2005-06-01
著者
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KAJIWARA Hideyuki
National Institute of Agrobiological Sciences
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ITO Yoko
National Institute of Agrobiological Sciences
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NAKAMURA Masatoshi
National Institute of Agrobiological Sciences
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MITA Kazuei
National Institute of Agrobiological Sciences
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IMAMAKI Atsue
Environmental Research Center
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ISHIZAKA Masami
National Institute for Agro-Environmental Sciences
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KAJIWARA Hideyuki
Department of Molecular Biology, National Institute of Agrobiological Resources
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Nakamura Masatoshi
Department of Pediatric Surgery, Reproductive and Developmental Medicine, Graduate School of Medical
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ITO Yoko
Department of Pediatrics, Kyoto Second Red Cross Hospital
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Kajiwara Hideyuki
Department Of Biochemistry National Institute Of Agrobiological Sciences
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IMAMAKI Atsue
Department of Biochemistry, National Institute of Agrobiological Sciences
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MITA Kazuei
Department of Genome Research, NIAS
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ISHIZAKA Masami
Department of Research Planning and Coordination, National Institute for Agro-Environmental Sciences
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Ito Yoko
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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Nakamura Masatoshi
Department Of Advanced Systems Control Engineering Saga University
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Nakamura Masatoshi
Department Of Advanced Systems Control Engineering Graduate School Of Science And Engineering Saga U
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