Proteome analysis of silkworm : 1. Fat body

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概要

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• Proteome analysis of silkworm (Bombyx mori L.) fat bodies was performed on the fifth-instar day-3 larva stage and the expression of proteins separated on two-dimensional polyacrylamide gels was tracked from the fourth-instar day-1 larva stage to the stage before adult moth. Proteins on the 2D-gels were excised manually and treated with 4-vinylpyridine for alkylation. Each spot was analyzed by capillary high-performance liquid chromatography coupled with ion-trap mass spectrometry after proteolysis using trypsin. In the previous report1), the amino acid sequence database obtained from the Drosophila genome was used for the protein identification. This analysis used the amino acid sequence data elucidated from the silkworm genome. Additionally, protein expression in fat bodies was compared with that at other stages and in other tissues described in the silkworm proteome database (http://kaiko2ddb.dna.affrc.go.jp/cgi-bin/search_2DDB.cgi). Several unidentified proteins from the previous report1) were identified with high Mascot scores. Induction and reduction of proteins during metamorphosis were observed as changes in spot concentration on sequential 2D-gels on the web.

• 2009-06-01

著者

• KAJIWARA Hideyuki

  National Institute of Agrobiological Sciences

• NAKAMURA Masatoshi

  National Institute of Agrobiological Sciences

• MITA Kazuei

  National Institute of Agrobiological Sciences

• IMAMAKI Atsue

  Environmental Research Center

• XIA Qingyu

  Institute of Sericulture and Systems Biology, Southwest University

• ISHIZAKA Masami

  National Institute for Agro-Environmental Sciences

• Xia Qingyu

  Institute Of Sericulture And Systems Biology Southwest University

• Kajiwara Hideyuki

  National Institute Of Agrobiological Resources

• Mita Kazuei

  National Institute Of Agrobiological Science

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