Proteome analysis of silkworm : 2. Hemolymph

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概要

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• Proteomic analysis of silkworm hemolymph at the fifth-instar day-3 larva stage was performed by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Prominent spots on 2D-gel were analyzed after trypsinization and proteins were identified by Mascot and Sequest using the amino acid sequence data obtained from the genome sequence of silkworm. The gel images were tracked from the fourth-instar day-1 larva stage to the stage before adult moth for twenty-one days. The results of mass spectrometry analysis and changes in gel images are summarized in the silkworm proteome database (http://kaiko2ddb.dna.affrc.go.jp/cgi-bin/search_2DDB.cgi).

• 2009-06-01

著者

• KAJIWARA Hideyuki

  National Institute of Agrobiological Sciences

• NAKAMURA Masatoshi

  National Institute of Agrobiological Sciences

• MITA Kazuei

  National Institute of Agrobiological Sciences

• IMAMAKI Atsue

  Environmental Research Center

• XIA Qingyu

  Institute of Sericulture and Systems Biology, Southwest University

• ISHIZAKA Masami

  National Institute for Agro-Environmental Sciences

• Xia Qingyu

  Institute Of Sericulture And Systems Biology Southwest University

• Kajiwara Hideyuki

  National Institute Of Agrobiological Resources

• Mita Kazuei

  National Institute Of Agrobiological Science

関連論文

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• Proteome analysis of silkworm : 2. Hemolymph
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