W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori
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概要
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We isolated four W chromosome-derived bacterial artificial chromosome (W-BAC) clones from Bombyx mori BAC libraries by the polymerase chain reaction and used them as probes for fluorescence in situ hybridization (FISH) on chromosome preparations from B. mori females. All four W-BAC probes surprisingly highlighted the whole wild-type W sex chromosome and also identified the entire original W-chromosomal region in W chromosome-autosome translocation mutants. This is the first successful identification of a single chromosome by means of BAC-FISH in species with holokinetic chromosomes. Genomic in situ hybridization (GISH) by using female-derived genomic probes highlighted the W chromosome in a similar chromosome-painting manner. Besides the W, hybridization signals of W-BAC probes also occurred in telomeric and/or subtelomeric regions of the autosomes. These signals coincided well with those of female genomic probes except one additional GISH signal that was observed in a large heterochromatin block of one autosome pair. Our results support the opinion that the B. mori W chromosome accumulated transposable elements and other repetitive sequences that also occur, but scattered, elsewhere in the respective genome.Edited by: E.R. Schmidt
- Springer-Verlag GmbHの論文
- 2003-06-24
著者
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佐原 健
Laboratory Of Applied Molecular Entomology Division Of Applied Bioscience Graduate School Of Agricul
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伴戸 久徳
Laboratory Of Applied Molecular Entomology Division Of Applied Bioscience Graduate School Of Agricul
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Shimada T
Department Of Agricultural And Environmental Biology Graduate School Of Agricultual And Life Science
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Abe H
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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阿部 弘亨
Univ. Of Tokyo
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阿部 弘亨
東京大学大学院工学系研究科原子力専攻
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Shimada Toru
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Shimada Toru
Department Of Agricultural And Environmental Biology Graduate School Of Agricultual And Life Science
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Sugasaki Toshiyuki
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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Abe Hiroaki
Department Of Biological Production Tokyo University Of Agriculture And Technology
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Abe Hiroaki
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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YASUKOCHI Yuji
National Institute of Agrobiological Sciences
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浅野 眞一郎
北海道大学大学院農学院 生物資源科学専攻 応用分子昆虫学分野
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Mita Kazuei
National Institute Of Agrobiological Science
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Shimada T
Nissan Motor Co. Ltd
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浅野 眞一郎
Laboratory Of Applied Molecular Entomology Department Of Applied Bioscience Research Faculty Of Agri
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Bando H
Faculty Of Agriculture Hokkaido University
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浅野 眞一郎
北海道大学大学院農学研究院
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Yoshido Atsuo
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Oshiki Toshikazu
Department of Agriculture, Tokyo University of Agriculture and Technology
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