Genetic mapping of RAPD markers linked to the densonucleosis refractoriness gene, nsd-1, in the silkworm, Bombyx mori
スポンサーリンク
概要
- 論文の詳細を見る
In the silkworm, Bombyx mori, nonsusceptibility to B. mori densonucleosis virus type-1 (BmDNV-1) is controlled by a recessive gene, nsd-1 (nonsusceptibility to DNV-1), located on the twenty-first chromosome. We investigated genetic linkage between five random amplified polymorphic DNA (RAPD) markers and the +nsd-1 gene. Initially, we constructed the CSD-1 strain (nsd-1/+) which is congenic to strain C137 (nsd-1/ nsd-1) for the twenty-first chromosome, starting with a female of C137 and a male of strain J137 (+nsd-1/+nsd-1). For the crossing over experiment, a female of C137 was crossed with a male (nsd-1/+) of CSD-1. Segregation analysis showed that the most closely linked RAPD marker mapped 3.0 cM distant from +nsd-1. A more specific marker for +nsd-1 was made by converting this RAPD band into a sequence characterized amplified region (SCAR) using a series of newly designed primer pairs based on its DNA sequence.
- 日本遺伝学会の論文
著者
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Shimada T
Department Of Agricultural And Environmental Biology Graduate School Of Agricultual And Life Science
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ABE Hiroaki
Department of Quantum Engineering and Systems Science, The University of Tokyo
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Abe H
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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阿部 弘亨
Univ. Of Tokyo
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阿部 弘亨
東京大学大学院工学系研究科原子力専攻
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Shimada Toru
Graduate School of Agricultural and Life Sciences, University of Tokyo
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KANEHARA Mariko
Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Tec
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OSHIKI Toshikazu
Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Tec
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HARADA Tae
Department of Biological Production, Faculty of Agriculture, Tokyo University
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Kanehara Mariko
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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Shimada Toru
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Sugasaki Toshiyuki
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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Abe Hiroaki
Department Of Biological Production Tokyo University Of Agriculture And Technology
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Abe Hiroaki
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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Shimada T
Nissan Motor Co. Ltd
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Ohbayashi Fumi
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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Harada Tae
Department Of Biological Production Faculty Of Agriculture Tokyo University
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Oshiki Toshikazu
Department of Agriculture, Tokyo University of Agriculture and Technology
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