Glycine addition improves feeding performance of non-specialist herbivores on the privet, Ligustrum obtusifolium: In vivo evidence for the physiological impacts of anti-nutritive plant defense with iridoid and insect adaptation with glycine
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概要
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The leaves of the privet tree, Ligustrum obtusifolium (Oleaceae), retain a strong lysine-decreasing activity caused by enzymatically-activated oleuropein, an iridoid glycoside. Protein treated with this activity become innutritive to insects because of the loss of lysine. We previously found that several privet specialist caterpillars secrete high concentrations of glycine in their digestive juices. Previous in vitro experiments showed that glycine inhibits lysine-decreasing activity of oleuropein. Therefore, we hypothesized that the lysine-decreasing activity acts to defend privet tree against herbivores and that glycine secretion by insects is an adaptive trait to counter the privet defense. In the present study, we aimed to examine whether these assumptions hold true under physiological conditions, and performed in vivo bioassays and physiological analyses using the Eri silkworm, Samia ricini (Saturniidae), a non-privet specialist. Significant decreases in larval growth and lysine concentration in the midgut lumen were observed when larvae were fed intact privet leaves compared to when they were fed heat inactivated privet leaves. These decreases were inhibited when larvae were fed intact privet leaves together with glycine, indicating that the privet defense with oleuropein and the specialist adaptation with glycine do function under physiological conditions. This study thus provides a rare view into the detailed physiological impacts of anti-nutritive plant defense and insect physiological adaptation in vivo.
- Japanese Society of Applied Entomology and Zoologyの論文
著者
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NAKAMURA Masatoshi
National Institute of Agrobiological Sciences
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KONNO Kotaro
National Institute for Agrobiological Sciences
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Hirayama Chikara
National Institute of Agrobiological Sciences
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Shinbo Hiroshi
National Institute of Agrobiological Sciences
関連論文
- Proteome analysis of silkworm : 3. Malpighian tube
- Proteome analysis of silkworm : 2. Hemolymph
- Proteome analysis of silkworm : 1. Fat body
- Draft of silkworm proteome database
- Defense characteristics of seral deciduous broad-leaved tree seedlings grown under differing levels of CO2 and nitrogen
- PURIFICATION AND CHARACTERIZATION OF ORNITHINE AMINOTRANSFERASE FROM THE FAT BODY OF SILKWORM, BOMBYX MORI(Biochemistry,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Hierarchical cluster analyses and heat map analyses of silkworm tissues using R-statistics
- Protein profile of silkworm midgut of fifth-instar day-3 larvae
- Proteomic Analysis of Silkworm Fat Body
- Glycine addition improves feeding performance of non-specialist herbivores on the privet, Ligustrum obtusifolium: In vivo evidence for the physiological impacts of anti-nutritive plant defense with iridoid and insect adaptation with glycine
- Selective absorption of mulberry leaf urease by the silkworm, Bombyx mori(Biochemistry)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Insect lipophorin and vertebrate lipoproteins support larval development of the endoparasitoid Venturia canescens (Hymenoptera : Ichneumonidae) as dietary lipid sources
- A Leaf-rolling Caterpillar Improves Leaf Quality(Behavior and Ecology)
- Defense characteristics of seral deciduous broad-leaved tree seedlings grown under differing levels of CO2 and nitrogen
- Reinstatement of the 27th linkage group in the classical linkage map of Bombyx mori by molecular linkage analysis of faint-green cocoon gene