An aromatic prenyltransferase-like gene HIPT-1 preferentially expressed in lupulin glands of hop
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概要
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Prenylated aromatic compounds represent the chemical components in the glandular trichomes (lupulin glands) of hops (Humulus lupulus, Cannabinaceae), which give the characteristic flavor and taste of beer. To isolate cDNAs for prenyltransferase recognizing aromatic substrates in hops, we constructed a cDNA library from the lupulin glands and randomly sequenced 11,233 EST clones, to obtain 6,613 non-redundant EST sequence information. Among them, we found an aromatic prenyltransferase-like gene (HlPT-1), which possessed three features of the plant aromatic prenyltransferase family, i.e., a D-rich motif, membrane-spanning domains, and a transit peptide. The tissue-specific expression study of HlPT-1 in the intact plant revealed this gene to be highly expressed in hop corns (female flowers), especially in the lupulin glands. Subcellular localization analysis using GFP fusion proteins suggested that HlPT-1 was localized to plastids. Phylogenetic analysis predicted that the HlPT-1 gene evolved from homogentisate prenyltransferases involved with vitamin E and plastoquinone biosynthesis.
- 2010-06-25
著者
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Tsurumaru Yusuke
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto Universi
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Sasaki Kanako
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto Universi
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Miyawaki Tatsuya
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto Universi
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Momma Takayuki
Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd.
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Umemoto Naoyuki
Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd.
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Yazaki Kazufumi
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto Universi
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Sasaki Kanako
Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University
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Momma Takayuki
Central Laboratories For Frontier Technology Kirin Holdings Co. Ltd.
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Umemoto Naoyuki
Central Laboratories For Frontier Technology Kirin Holdings Co. Ltd.
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Umemoto Naoyuki
Central Lab. Key Tech. Kirin Brewery Co.
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Yazaki Kazufumi
Laboratory Of Gene Expression Wood Research Institute Kyoto University
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Miyawaki Tatsuya
Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University
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Tsurumaru Yusuke
Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University
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UMEMOTO Naoyuki
Central Lab. Key Tech. , Kirin Brewery Co.
関連論文
- An aromatic prenyltransferase-like gene HlPT-1 preferentially expressed in lupulin glands of hop
- An aromatic prenyltransferase-like gene HIPT-1 preferentially expressed in lupulin glands of hop
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