Characterization of Coumarin-Specific Prenyltransferase Activities in Citrus limon Peel

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概要

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• Coumarins, a large group of polyphenols, play important roles in the defense mechanisms of plants, and they also exhibit various biological activities beneficial to human health, often enhanced by prenylation. Despite the high abundance of prenylated coumarins in citrus fruits, there has been no report on coumarin-specific prenyltransferase activity in citrus. In this study, we detected both O- and C-prenyltransferase activities of coumarin substrates in a microsome fraction prepared from lemon (Citrus limon) peel, where large amounts of prenylated coumarins accumulate. Bergaptol was the most preferred substrate out of various coumarin derivatives tested, and geranyl diphosphate (GPP) was accepted exclusively as prenyl donor substrate. Further enzymatic characterization of bergaptol 5-O-geranyltransferase activity revealed its unique properties: apparent Km values for GPP (9 µM) and bergaptol (140 µM) and a broad divalent cation requirement. These findings provide information towards the discovery of a yet unidentified coumarin-specific prenyltransferase gene.

著者

• Sasaki Kanako

  Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University

• Azuma Jun-ichi

  Division Of Environmental Science And Technology Graduate School Of Agricultural Science Kyoto Unive

• Yazaki Kazufumi

  Laboratory Of Gene Expression Wood Research Institute Kyoto University

• Tsurumaru Yusuke

  Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University

• Hori Hitoshi

  Department Of Biochemistry And Molecular Biology Nippon Medical School

• Sugiyama Akifumi

  Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University

• Uto Yoshihiro

  Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima

• INOUE Tsuyoshi

  Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University

• KOEDUKA Takao

  Institute for Chemical Research, Kyoto University

• Munakata Ryosuke

  Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University

• Inoue Tsuyoshi

  Division Of Environmental Science And Technology Graduate School Of Agriculture Kyoto University

• Uto Yoshihiro

  Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima

• Sasaki Kanako

  Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University

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