Galactinol Synthase Gene of Coptis japonica Is Involved in Berberine Tolerance
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概要
- 論文の詳細を見る
Many plant secondary metabolites show strong biological activities and are potentially also toxic to plants, while plants producing such active compounds are usually insensitive to their own metabolites, suggesting that they have species-specific detoxification mechanisms. In order to clarify the detoxification mechanism of alkaloids, we used cultured cells of Coptis japonica, which are capable of producing a yellow benzylisoquinoline alkaloid, berberine, and accumulate it in the vacuole. Unlike other plant cells that do not produce berberine, C. japonica shows strong tolerance to this alkaloid. We established a fission yeast strain that was sensitive to berberine and performed functional screening using a C. japonica cDNA library. One cDNA clone, which conferred clear berberine tolerance, encoded galactinol synthase (CjGolS). The possible role of CjGolS in berberine tolerance is discussed.
- 社団法人 日本農芸化学会の論文
- 2008-02-23
著者
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Yazaki Kazufumi
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto Universi
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Yazaki Kazufumi
Kyoto Univ. Kyoto Jpn
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TAKEGAWA Kaoru
Department of Life Science, Faculty of Agriculture, Kagawa University
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Iwaki Tomoko
Department Of Biological Resources Faculty Of Agriculture Ehime University
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Yazaki Kazufumi
Lab. Of Plant Gene Expression Res. Inst. For Sustainable Humanosphere Kyoto Univ.
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Yazaki Kazufumi
Laboratory Of Gene Expression Wood Research Institute Kyoto University
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HAMAMOTO Masafumi
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto Universi
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SHITAN Nobukazu
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto Universi
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KAMIMOTO Yoshihisa
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto Universi
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Takanashi Kojiro
Laboratory Of Plant Gene Expression Rish Kyoto University
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Kamimoto Yoshihisa
Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University
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Takegawa Kaoru
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios
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Shitan Nobukazu
Laboratory Of Molecular And Cellular Biology Of Totipotency Division Of Integrated Life Science Grad
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Takanashi Kojiro
Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University
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SUGIYAMA Akifumi
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto Universi
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Sugiyama Akifumi
Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University
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Hamamoto Masafumi
Laboratory Of Plant Gene Expression Research Institute For Sustainable Humanosphere Kyoto University
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Shitan Nobukazu
Department Of Natural Medicinal Chemistry Kobe Pharmaceutical University
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IWAKI Tomoko
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University
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