Transfer of High-Mannose-Type Oligosaccharides to Disaccharides by Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae

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概要

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• Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) has transglycosylation activity, and high-mannose-type oligosaccharides are transferred to suitable glycosides as acceptor substrates. The acceptor specificity of Endo-A-catalyzed transglycosylation toward various disaccharides was investigated. To identify an effective acceptor for the transglycosylation by Endo-A, the reaction was carried out using various disaccharides. Endo-A transferred high-man-nose-type oligosaccharides more efficiently to β-linked disaccharides (cellobiose, gentiobiose, sophorose, and laminaribiose) than to α-linked disaccharides (isomaltose, maltose, nigerose, kojibiose, and trehalose) as acceptor substrates. The transglycosylation products, (Man)_6GlcNAc-Glc-β-Glc, were more rapidly hydrolyzed than (Man)_6GlcNAc-Glc-α-Glc. These results indicate that Endo-A recognizes the anomerlic configuration of the acceptor substrates, and β-linked glycosides are suitable for the synthesis of transglycosylation products.

• 社団法人日本生物工学会の論文
• 2002-06-20

著者

• Fujita Kiyotaka

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Fujita Kiyotaka

  Department Of Lfe Sciences Faculty Of Agriculture Kagawa University:(present Address)division Of Int

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Takegawa Kaoru

  Department Of Lfe Sciences Faculty Of Agriculture Kagawa University

• Kato Ikunoshin

  Biotechnology Research Laboratories Takara Bio Inc.

• Sano Mutsumi

  Biotechnology Research Laboratories Takara Shuzo Co. Ltd.

• Sano Mutsumi

  Biotechnology Research Laboratory Takara Shuzo Co. Ltd.

• Kato Ikunoshin

  Biotechnology Research Laboratory Takara Shuzo Co. Ltd.

• MIYAMURA TSUYOSHI

  Biotechnology Research Laboratory, Takara Shuzo Co. Ltd.

• Miyamura Tsuyoshi

  Biotechnology Research Laboratory Takara Shuzo Co. Ltd.

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