Transfer of High-Mannose-Type Oligosaccharides to Disaccharides by Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae
スポンサーリンク
概要
- 論文の詳細を見る
Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) has transglycosylation activity, and high-mannose-type oligosaccharides are transferred to suitable glycosides as acceptor substrates. The acceptor specificity of Endo-A-catalyzed transglycosylation toward various disaccharides was investigated. To identify an effective acceptor for the transglycosylation by Endo-A, the reaction was carried out using various disaccharides. Endo-A transferred high-man-nose-type oligosaccharides more efficiently to β-linked disaccharides (cellobiose, gentiobiose, sophorose, and laminaribiose) than to α-linked disaccharides (isomaltose, maltose, nigerose, kojibiose, and trehalose) as acceptor substrates. The transglycosylation products, (Man)_6GlcNAc-Glc-β-Glc, were more rapidly hydrolyzed than (Man)_6GlcNAc-Glc-α-Glc. These results indicate that Endo-A recognizes the anomerlic configuration of the acceptor substrates, and β-linked glycosides are suitable for the synthesis of transglycosylation products.
- 社団法人日本生物工学会の論文
- 2002-06-20
著者
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Fujita Kiyotaka
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Fujita Kiyotaka
Department Of Lfe Sciences Faculty Of Agriculture Kagawa University:(present Address)division Of Int
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Takegawa Kaoru
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios
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Takegawa Kaoru
Department Of Lfe Sciences Faculty Of Agriculture Kagawa University
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Kato Ikunoshin
Biotechnology Research Laboratories Takara Bio Inc.
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Sano Mutsumi
Biotechnology Research Laboratories Takara Shuzo Co. Ltd.
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Sano Mutsumi
Biotechnology Research Laboratory Takara Shuzo Co. Ltd.
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Kato Ikunoshin
Biotechnology Research Laboratory Takara Shuzo Co. Ltd.
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MIYAMURA TSUYOSHI
Biotechnology Research Laboratory, Takara Shuzo Co. Ltd.
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Miyamura Tsuyoshi
Biotechnology Research Laboratory Takara Shuzo Co. Ltd.
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