Purification and Characterization of a Novel Lyase from Cellulomonas sp. that Degrades Fusarium and Gibberella Acidic Polysaccharides(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• A Cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of Fusarium sp. M7-1 with the formation of mannose and O-β-D-mannopyranosyl-(1→2)-D-mannose. DEAE-Toyopearl 650M column chromatography showed three lyase activity peals (fractions I, II, and III). The major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. The optimum pH was 6.5 to 8.0 and the stable pH range was 6.0 to 8.0. The purified enzyme did not degrade glucuronic or galacturonic acid-containing polysaccharides such as chondroitin, hyaluronic acid, pectin, or pectic acid. However, the purified enzyme specifically degraded various Fusarium and Gibberella acidic polysaccharides, and unsaturated sugars were produced with the release of mannose and O-β-D-mannopyranosyl-(1→2)-D-mannose. These results suggest that the acidic polysaccharides derived from Fusarium and Gibberella have similar structures.

• 社団法人日本農芸化学会の論文
• 1991-08-23

著者

• YAMAGUCHI Shinya

  Department of Neurosurgery, Shi-Koga Hospital

• TAKEGAWA Kaoru

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Takegawa Kaoru

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa K

  Kagawa Univ. Kagawa Jpn

• JIKIBARA TAKAYUKI

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• IWAHARA SHOJIRO

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• MIKI Satoshi

  Department of Physics, Kyoto University

• Iwahara S

  Kagawa Univ. Kagawa Jpn

• Iwahara Shojiro

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Miki Satoshi

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Yamaguchi S

  Kanagawa Univ. Yokohama Jpn

• Iwahara Shojiro

  Department Of Agricultural Chemistry Faculty Of Agriculture Kagawa University

• Yamaguchi Shinya

  Department Of Environment And Forest Resources Science Faculty Of Agriculture Shizuoka University

• Jikibara Takayuki

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Yamaguchi Shinya

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

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