Isolation and Characterization of a Glycosylation Mutant from Schizosaccharomyces pombe
スポンサーリンク
概要
- 論文の詳細を見る
N-Linked oligosaccharides were elongated by glycosylation with mannose and galactose residues in the secretory pathway of Schizosaccharomyces pombe. The wild-type S. pombe cells were agglutinated by the additions of not only concanavalin A lectin, which is specific for mannose residues, but also PNA (from Arachis hypogaea) and RCA (Ricinus communis) lectins, which are specific for terminal galactose residues. By PNA-binding selection, we isolated an S. pombe mutant defective in protein glycosylation. The mutant cells, named gms1, were not agglutinated by PNA or RCA. In contrast, agglutination of the gms1 cells by the addition of concanavalin A was markedly increased. Structural studies on N-linked oligosaccharides from gms1 mutant cells showed that the number of α-1,2-linked galactose residues was markedly reduced, and unsubstituted α-1,6-linked polymannose outer chains were attached to the core oligosaccharides.
- 社団法人日本農芸化学会の論文
- 1996-07-23
著者
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Tanaka Naotaka
Department of Life Science, Kagawa University
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TAKEGAWA Kaoru
Department of Life Science, Faculty of Agriculture, Kagawa University
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Takegawa Kaoru
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Takegawa K
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Takegawa Kaoru
Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.
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Takegawa K
Kagawa Univ. Kagawa Jpn
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IWAHARA SHOJIRO
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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TABUCHI Mitsuaki
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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Iwahara S
Kagawa Univ. Kagawa Jpn
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Iwahara Shojiro
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Takegawa K
Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.
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Takegawa Kaoru
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios
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Tabuchi Mitsuaki
Yonezawa Women's College Of Yamagata Prefecture
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Tanaka Naotaka
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Iwahara Shojiro
Department Of Agricultural Chemistry Faculty Of Agriculture Kagawa University
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