Chemo-Enzymatic Synthesis of a Calcitonin Derivative Containing a High-Mannose Type Oligosaccharide by Endo-β-N-Acetylglucosaminidase from Arthrobacter protophormiae

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概要

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• Chemo-enzymatic addition of a high-mannose type oligosaccharide to eel calcitonin (CT), a calciumregulating hormone, was examined. The endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) transglycosylated the Man_6-GlcNAc moiety from an ovalbumin-derived high-mannose type glycosyl asparagine, Asn(Man_6-GlcNAc_2)-OH, to a calcitonin derivative, [Asn(GlcNAc)^3]-CT, in which the N-acetyl-D-glucosamine (GlcNAc) is attached to the third L-asparagine (Asn) residue of the peptide, and a calcitonin derivative containing a high-mannose type oligosaccharide, [Asn(Man_6-GlcNAc_2)^3]-CT, was synthesized. The optimal reaction conditions for the synthesis of [Asn(Man_6-GlcNAc_2)^3]-CT from Asn(Man_6-GlcNAc_2)-OH and [Asn(GlcNAc)^3]-CT catalyzed by Endo-A were examined. The transglycosylation yield relative to the concentration of the [Asn(GlcNAc)^3]-CT added was 32.7%, and 4.42 mg of [Asn(Man_6-GlcNAc_2)^3]-CT was prepared.

• 社団法人日本生物工学会の論文
• 1999-02-25

著者

• KATO Ikunoshin

  Biotechnology Research Laboratories, Takara Bio Inc.

• Inazu Toshiyuki

  The Noguchi Institute

• Mizuno Mamoru

  The Noguchi Institute

• TAKEGAWA Kaoru

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Takegawa K

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa K

  Kagawa Univ. Kagawa Jpn

• Yamamoto K

  Graduate School Of Biostudies Kyoto University

• Mizuno M

  Noguchi Inst. Tokyo Jpn

• Mizuno M

  Department Of Energy Science And Engineering Kyoto University

• Haneda Katsuji

  The Noguchi Inst.

• 羽田 勝二

  (財)野口研究所糖質酵素化学研究室

• YAMAMOTO Keizo

  The Noguchi Institute

• IGUCHI REIKO

  The Noguchi Institute

• KONDO AKIHIKO

  Biotechnology Research Laboratory, Takara Shuzo Co.Ltd.,

• Takegawa K

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Kato Ikunoshin

  Biotechnology Research Laboratories Takara Bio Inc.

• Kato I

  Takara Shuzo Co. Ltd. Shiga Jpn

• Mizuno M

  The Noguchi Institute

• Kato Ikunoshin

  Biotechnology Research Laboratory Takara Shuzo Co.ltd.

• 稲津 敏行

  東海大学工学部応用化学科

• Kondo Akihiko

  Biotechnology Research Laboratory Takara Shuzo Co.ltd.

• Igychi Reiko

  The Noguchi Institute

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