Isolation of Suppressor Mutants of Phosphatidylinositol 3-Phosphate 5-kinase Deficient Cells in Schizosaccharomyces pombe(Microbiology & Fermentation Technology)

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概要

• 論文の詳細を見る

• The ste12^+ gene of Schizosaccharomyces pombe codes for a phosphatidylinositol (PI) 3-phosphate 5'-kinase, which is required for efficient mating. Suppressor mutants for sterility of ste12Δ cells were screened for. Most of the mutant genes turned out to be recessive. Six genes were cloned and the open reading frames responsible for the suppressor activity were identified. They included genes coding for proteins with domains homologous to calcium transporters, casein kinase II, UBC13, AMSH, Vps23p, and Vps27p of Saccharomyces cerevisiae. Disruption of these genes resulted in suppression of the defects of the ste12Δ cells, including low mating efficiency and formation of large vacuoles. Since many of these gene products are homologous to the proteins involved in vesicle transport, sterility caused by inactivation of ste12 may be due to a disordered vesicle transport system.

• 社団法人日本農芸化学会の論文
• 2003-08-23

著者

• TAKEGAWA Kaoru

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Takegawa K

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa K

  Kagawa Univ. Kagawa Jpn

• Nakamura Yoko

  Laboratory Of Biological Chemistry Graduate School Of Agricultural And Life Science University Of To

• FUKUI Yasuhisa

  Laboratory of Biological Chemistry, Division of Applied Biological Chemistry, Graduate School of Agr

• Nakamura Y

  Department Of Biofunctional Chemistry Division Of Bioscience Graduate School Of Natural Science And

• Takegawa K

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• ONISHI Masayuki

  Laboratory of Biological Chemistry, Graduate School of Agricultural and Life Science, University of

• KOGA Takako

  Laboratory of Biological Chemistry, Graduate School of Agricultural and Life Science, University of

• Fukui Y

  Research Center For Environmental Genomics Kobe University

• Onishi Miki

  Laboratory Of Biological Chemistry Graduate School Of Agricultural And Life Science University Of To

• Fukui Yasuhisa

  Laboratory Of Biological Chemistry Division Of Applied Biological Chemistry Graduate School Of Agric

• Fukui Yuko

  Institute For Fundamental Research Suntory Ltd. (sunbor)

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