Effect of Deglycosylation of Polymannose Chains on the Properties of Yeast External Invertase

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概要

• 論文の詳細を見る

• α-Mannosidase from Cellulomonas sp. released about 70% of the polymannose chains from yeast external invertase. Native and mannose-depleted yeast invertases were compared with regard to various enzymatic properties. It was found that their catalytic activity and thermal and pH stability were identical. However, the man-nose-depleted invertase was more sensitive to proteinases such as pronase and subtilisin. The mannose depleted invertase was less stable than the native enzyme when incubated with sodium dodecyl sulfate. These results show that the polymannose chains of yeast invertase contribute to the high stability of the enzyme.

• 社団法人日本生物工学会の論文
• 1990-08-25

著者

• TAKEGAWA Kaoru

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Takegawa Kaoru

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa K

  Kagawa Univ. Kagawa Jpn

• IWAHARA SHOJIRO

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• MIKI Satoshi

  Department of Physics, Kyoto University

• Iwahara S

  Kagawa Univ. Kagawa Jpn

• Iwahara Shojiro

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Miki Satoshi

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Iwahara Shojiro

  Department Of Agricultural Chemistry Faculty Of Agriculture Kagawa University

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