Purification and Characterization of Endo-β-N-acetylglucosaminidase from a Flavobacterium sp.
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概要
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A gram negative bacterium isolated from soil was found to produce a high level of endo-β-N-acetylglucosaminidase in the culture medium. The organism was identified as a Flavobacterium sp. from various bacteriological characteristics. The enzyme from the Flavobacterium sp. was purified to homogeneity from culture broth by fractionation with ammonium sulfate and column chromatographies on DEAE-cellulose, hydroxylapatite, and Sephadex G-150 and G-100. The molecular weight of the enzyme was estimated to be 27, 000 and 30, 000 by gel filtration and SDS-polyacrylamide gel electrophoresis, respectively, and it appeared to consist of a single polypeptide chain. The optimal pH for activity was 5.0 to 6.0 and the stable pH range was 5-7. The Michaelis constant was 0.30 mM with dansyl-Asn-(GlcNAc)2(Man)6 as the substrate. The enzyme hydrolyzed oligosaccharides of native ovalbumin, bovine pancreatic ribonuclease B and a yeast invertase.
- 社団法人 日本農芸化学会の論文
著者
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KADOWAKI SETSU
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kacho Junior Co
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Tochikura Tatsurokuro
Department Of Agricultural Chemistry Kyoto University
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Takegawa Kaoru
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios
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KUMAGAI Hidehiko
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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KADOWAKI Setsu
Department of Food Science and Technology, Kyoto University
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TAKEGAWA Kaoru
Department of Food Science and Technology, Kyoto University
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YAMAMOTO Kenji
Kacho College
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