A Set of loxP Marker Cassettes for Cre-mediated Multiple Gene Disruption in Schizosaccharomyces pombe

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概要

• 論文の詳細を見る

• For functional analysis, the presence of gene families and isoenzymes often makes it necessary to delete more than one gene, while the number of marker genes is limited in Schizosaccharomyces pombe. Here we describe a loxP-flanked ura4+ cassette and Cre recombinase vector for a Cre-loxP-mediated marker removal procedure in S. pombe. This loxP-ura4-loxP cassette can be used for disruption of hmt1+ as a model target gene. We have constructed two vectors which express Cre recombinase under the control of the nmt1 or nmt41 promoter. Excisive recombination at loxP sites in the chromosome was promoted efficiently and accurately when the Cre recombinase was expressed under the control of the nmt41 promoter. In addition, ura4+ could be excised from the genome by Cre recombinase, when a single loxP site was adjacent to ura4. The use of the Cre-loxP system proved to be a practical strategy to excise a marker gene for repeated use in S. pombe.

• 社団法人 日本農芸化学会の論文
• 2004-03-23

著者

• TAKEGAWA Kaoru

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Iwaki T

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Iwaki Tomoko

  Department Of Biological Resources Faculty Of Agriculture Ehime University

• Iwaki Tomoko

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• IWAKI Tomoko

  Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University

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