Schizosaccharomyces pombe Pep12p is required for vacuolar protein transport and vacuolar homotypic fusion(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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概要

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• In eukaryotic cells, SNARE proteins are essential for intracellular vesicle trafficking. Several SNARE proteins are required for vacuolar protein transport and vacuolar biogenesis in Saccharomyces cerevisiae. Previously we demonstrated that one of the fission yeast SNARE proteins, Pep12p, is not required for vacuolar fusion process in Schizosaccharomyces pombe. We have re-examined the function of S. pombe Pep12p using the newly created pep12^+ deletion strain. Deletion of the fission yeast pep12^+ gene results in pleiotropic phenotypes consistent with the absence of normal vacuoles, including missorting of vacuolar carboxypeptidase Y-and various ion- and drug-sensitivities. GFP-Pep12 fusion protein is mostly localized at the vacuolar membrane and the prevacuolar compartment. The S. pombe pep12Δ mutation phenocopies that of vps33Δ, suggesting that both Pep12p and Vps33p act at the same membrane fusion step in S. pombe, and both mutations cause vacuolar deficiency.

著者

• HOSOMI Akira

  Department of Life Sciences, Faculty of Agriculture, Kagawa University

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Hosomi Akira

  Department Of Chemistry And Graduate School Of Chemistry University Of Tsukuba

• Hosomi Akira

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Hosomi Akira

  Department Of Life Sciences Faculty Of Agriculture Kagawa University:the Glycometabolome Team Riken

• Nakase Mai

  Laboratory of Applied Microbiology, Graduate School of Bioresource and Bioenvironmental Sciences, Ky

• Nakase Mai

  Laboratory Of Applied Microbiology Graduate School Of Bioresource And Bioenvironmental Sciences Kyus

• Takegawa Kaoru

  Department Of Life Sciences Faculty Of Agriculture Kagawa University:laboratory Of Applied Microbiol

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