Production and Characterization of Extracellular Uronic Acid-Containing Glycoproteins from Fusarium oxysporum

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概要

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• Fusarium and Giberalla species were found to secrete acidic polysaccharides when grown in Czapex-Dox medium. Fusarium oxysporum showed the highest rate of secretion of acidic sugars into the growth medium.The acidic sugar-containing glycoprotein from F. oxysporum was purified from the culture filtrate to apparrent homogeneity on ultracentrifugation analysis, by ammonium sulfate precipitation, Toyopearl HW-55 gel filtration and charcoal chromatography. The purified acidic sugar-containing glycoprotein has a 10% protein content and a content of 40% acidic sugars as glucuronic acid, and the neutral sugars present in this glycoprotein are mainly mannose, galactose, and glucose. The protein moiety of the glycoprotein contains a high proportion of serine and threonine residues. Treatment of the glycoprotein with alkaline solution resulted in an increase in absorbance at 241 nm, and alkaline borohydride treatment resulted in a marked decrease in the number of serine and threonine residues. We conclude that the glycoprotein has O-linked sugar chains which are mainly attached to serine and threonine residues of the protein moiety. The primary structure of the acidic polysaccharide from F. oxysporum was analyzed mainly by NMR spectrometry. The main parts of this polysaccharide have structures similar to those of uronic acid-containing polysaccharide from Fusarium sp. M7-1.

• 社団法人日本生物工学会の論文
• 1997-02-25

著者

• TAKEGAWA Kaoru

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Takegawa Kaoru

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa K

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa K

  Kagawa Univ. Kagawa Jpn

• SATOH KAZUNARI

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• RAMLI NAHROWI

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• JIKIBARA TAKAYUKI

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• IWAHARA SHOJIRO

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• Iwahara S

  Kagawa Univ. Kagawa Jpn

• Iwahara Shojiro

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa K

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Ramli Nahrowi

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Satoh Kazunari

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Iwahara Shojiro

  Department Of Agricultural Chemistry Faculty Of Agriculture Kagawa University

• Jikibara Takayuki

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

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