Degradation of β1→6 Galactofuranoside Linkages in the Polysaccharide of Fusarium sp. M7-1 by Endo-β-galactofuranosidase from Bacillus sp.

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概要

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• A polysaccharide, in which the main part of the side chains were depleted, was prepared from the acidic polysaccharides of Fusarium sp. M7-1 by digestion with lyase of Cellulomonas sp. and mild acid treatment. This polysaccharide was degraded into several fragments, neutral oligosaccharides, neutral polysaccharide, and acidic polysaccharide, by an enzyme, endo-β-galactofuranosidase, produced by Bacillus sp. The main components of the oligosaccharides were isolated and identified as Galfβ1→6Gal, Glcα1→2Galfβ1→6Gal, Glcα1→2Galfβ1→6Galfβ1→6Gal, Glcα1→2Galfβ1→(6Glcα1→2)Galfβ1→6Gal and Glcα1→2Galfβ1→6(Galfβ1→2)Galβ1→6Galβ1→6Gal. The molecular mass of the neutral polysaccharide fragment was estimated to be about 6000 Da by gel filtration chromatography. The polysaccharide fragment consisted of an α1→6 linked mannan main chain to which various sugars, namely Glc, Man, and Rha were attached through α1→3 (or 2) linkages. The molecular mass of the acidic polysaccharide fragment was estimated to be about 6000 Da from the amounts of the reducing terminal galactose. The chemical structures of the oligosaccharides derived from the acidic polysaccharide fragment by mild acid hydrolysis were identified as reported structural units [Iwahara et al., J. Biochem., 112, 355-359 (1992)]. The structure of the mild-acid-resistant part of the acidic polysaccharide fragment was assumed to be a polyuronide to which various sugars such as Glc, Man, and GlcNAc are attached as the side chains. The linkage modes of each sugar are not clear.

• 社団法人日本農芸化学会の論文
• 1996-06-23

著者

• TAKEGAWA Kaoru

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Takegawa Kaoru

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa K

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa K

  Kagawa Univ. Kagawa Jpn

• RAMLI NAHROWI

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• IWAHARA SHOJIRO

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• MISHIMA Toshihiro

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• Iwahara S

  Kagawa Univ. Kagawa Jpn

• Iwahara Shojiro

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa K

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Ramli Nahrowi

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Iwahara Shojiro

  Department Of Agricultural Chemistry Faculty Of Agriculture Kagawa University

• Mishima Toshihiro

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

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