CUE Domain-Containing Protein Vps901 Is Required for Vacuolar Protein Transport in <I>Schizosaccharomyces pombe</I>

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概要

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• The functions of two <I>Schizosaccharomyces pombe</I> Vps9-like genes, SPBC4F6.10/<I>vps901</I><SUP>+</SUP> and SPBC29A10.11c/<I>vps902</I><SUP>+</SUP>, were characterized. Genomic sequence analysis predicted that Vps901p contains a VPS9 domain, whereas cDNA analyses revealed that Vps901p contains a CUE domain (coupling of ubiquitin to ER degradation) in its C-terminal region. Deletion of <I>vps901</I><SUP>+</SUP> resulted in mis-sorting and secretion of <I>S. pombe</I> vacuolar carboxypeptidase Cpy1p, whereas deletion of <I>vps902</I><SUP>+</SUP> had no effect, suggesting that only Vps901p functions in vacuolar protein transport in <I>S. pombe</I>. Deletion of <I>vps901</I><SUP>+</SUP> further produced pleiotropic phenotypes, including vacuolar homotypic fusion and endocytosis defects. Heterologous expression of the budding yeast <I>VPS9</I> gene corrected the CPY mis-sorting defect in <I>vps901</I>Δ cells. These findings suggest that the VPS9 domain of Vps901p is required for vacuolar protein trafficking in <I>S. pombe</I>.

著者

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Hosomi Akira

  Department Of Chemistry And Graduate School Of Chemistry University Of Tsukuba

• Miyamoto Masaaki

  Department Of Applied Chemistry Muroran Institute Of Technology

• Nakase Mai

  Laboratory Of Applied Microbiology Graduate School Of Bioresource And Bioenvironmental Sciences Kyus

• Tsukamoto Yuta

  Department Of Biology Graduate School Of Science Kobe University

• NAKASE Mai

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University

• MATSUDA Tomo

  Department of Biology, Graduate School of Science, Kobe University

• Nakase Mai

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu University

• Matsuda Tomo

  Department Of Biology Graduate School Of Science Kobe University

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