Nystatin Effects on Vacuolar Function in Saccharomyces cerevisiae

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概要

• 論文の詳細を見る

• The effects of nystatin, a polyene antibiotic, was studied in Saccharomyces cerevisiae by isolating and characterizing nystatin-sensitive mutants. We isolated a number of nystatin-sensitive mutants by ethylmethane sulfonate mutagenesis. One of these mutants, the nss1 mutant, was characterized in detail. The mutant was sensitive to stresses such as high temperature or high concentrations of monovalent and divalent cations. The nss1 mutants showed severe vacuolar protein sorting and vacuolar morphology defects. The nss1 mutant was demonstrated to have a mutational lesion in the known VPS16 gene, which is essential for vacuolar protein sorting in S. cerevisiae. All of the vacuolar deficient mutants (vps11,vps16,vps18,and vps33) were sensitive to nystatin. Nystatin was found to cause extensive enlargement of the vacuole in wild-type S. cerevisiae cells. These results are discussed with special reference to the vacuolar function of S. cerevisiae.

• 社団法人日本農芸化学会の論文
• 1999-06-23

著者

• FUKUI HIROSHI

  Department of Biopharmaceutics, Kyoto Pharmaceutical University

• Fujita Kiyotaka

  Faculty Of Agriculture Kagoshima University

• Fujita Kiyotaka

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Fujita Kiyotaka

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Ito Yuji

  Department Of Bioengineering Faculty Of Engineering Kagoshima University

• Takegawa Kaoru

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Tanaka Naotaka

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Tanaka Naotaka

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Nakamura Atsuko

  Department Of Chemistry And Materials Science Tokyo Institute Of Technology

• BHUIYAN ShahAlamM.

  Department of Life Sciences, Faculty of Agriculture, Kagawa University

• Bhuiyan Shahalamm.

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Fukui Hiroshi

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University

• Nakamura Atsuko

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Ito Yuji

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Bhuiyan M.Shah

  Department of Life Sciences, Faculty of Agriculture, Kagawa University

• Nakamura Atsuko

  Department of Biochemistry, Kyoto Prefectural University of Medicine

• Ito Yuji

  Department of Appleed Chemiaity and Bialownology,Facutory of Enginiaring,niigata Institute of Technology

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