Mechanism of IgA-albumin complex formation that affects the fructosamine assay
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概要
- 論文の詳細を見る
We recently demonstrated glycation of monoclonal IgA and the presence of IgA-albumin complexes, but the mechanism of IgA-albumin complex formation was not clear. We isolated the IgA-albumin complexes from 5 IgA type M-proteinemia patients’ sera. To elucidate the mechanism of IgA-albumin complex formation, we performed the dissociation assay of IgA-albumin complexes, the identification of albumin binding sites of monoclonal IgA using immunoelectrophoresis, western blotting and chromatography technologies. In all patients with IgA type M-proteinemia, the IgA-albumin complexes were dissociated by treated with 2-mercaptoethanol (2-ME), but not by treated with a strong acid as acetic acid or NaCl of high concentrations. Moreover, when the purified monoclonal IgA containing IgA-albumin complexes was digested with the IgA protease from Neisseria gonorrhoeae, no macro-albumin was demonstrated. It seems probable that albumin is bound to the monoclonal IgA molecule by covalent disulfide bonds, and that the binding site of albumin is located in near the hinge region of IgA molecule and involve the free SH group thought to be present in the α-chain.
- 日本電気泳動学会の論文
- 2006-06-01
著者
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Terasawa Fumiko
Departments Of Biomedical Laboratories Sciences Shinshu University
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Terasawa Fumiko
Shinshu Univ. Jpn
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Terasawa Fumiko
Department Of Biomedical Laboratory Sciences School Of Health Sciences Shinshu University
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Okumura Nobuo
Shinshu Univ. Jpn
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KATO Yoko
Department of Neurosurgery, Fujita Health University
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KAMEKO Fumiko
Department of Medical Technology, School of Allied Medical Sciences, Shinshu University
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FUJITA Kiyotaka
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University
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FUKUSHIMA Mana
Department of Laboratory Medicine, Shinshu University School of Medicine
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OKUMURA Nobuo
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University
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SUGANO Mitsutoshi
Department of Laboratory Medicine, Shinshu University School of Medicine
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YAMAUCHI Kazuyoshi
Department of Laboratory Medicine, Shinshu University School of Medicine
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SATO Hirohisa
Department of Clinical Laboratories, Aomori Prefectural Central Hospital
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KAMEKO Mitsuaki
Department of Clinical Laboratories, Nagano Municipal Hospital
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SAKURABAYASHI Ikunosuke
Department of Clinical Laboratories, Omiya Medical Center, Jichi Medical School
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Fujita Kiyotaka
Chiba Inst. Sci. Choshi Jpn
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Fujita Kiyotaka
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Kato Yoko
Department Of Neurosurgery Fujita Health University
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Kameko Fumiko
Department Of Biomedical Laboratory Sciences School Of Health Sciences Shinshu University
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Kameko Fumiko
Shinshu Univ. Matsumoto Jpn
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Sato Hirohisa
Department Of Clinical Laboratories Aomori Prefectural Central Hospital
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Fukushima Mana
Department Of Laboratory Medicine Shinshu University School Of Medicine
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Sakurabayashi Ikunosuke
Department Of Clinical Laboratories Omiya Medical Center Jichi Medical School
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Kameko Mitsuaki
Department Of Clinical Laboratories Nagano Municipal Hospital
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Yamauchi Kazuyoshi
Department Of Laboratory Medicine Shinshu University Hospital
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Sugano Mitsutoshi
Department Of Laboratory Medicine Shinshu University School Of Medicine
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Kato Yoko
Department Of Materials Science Shizuoka Institute Of Science And Technology
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Kato Yoko
Department Of Biomedical Laboratory Sciences School Of Health Sciences Shinshu University
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Okumura Nobuo
Department Of Health And Medical Sciences Graduate School Of Medicine Shinshu University
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Okumura Nobuo
Department Of Biomedical Laboratory Sciences School Of Health Sciences Shinshu University
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Sugano Mitsutoshi
Department Of Laboratory Medicine Shinshu University Hospital
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