A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
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概要
- 論文の詳細を見る
The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR markedly reduced the reaction time of reverse transcription-PCR while maintaining the same sensitivity as conventional real-time PCR, and it can be used as a rapid assay for detection of BRSV. Furthermore, our method is potentially applicable for rapid diagnosis of almost all infectious diseases, including highly pathogenic avian influenza virus.
著者
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Honda Takayuki
Division Of Diagnostic Pathology Department Of Pathology And Laboratory Medicine Kariya General Hosp
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Matsuda Kazuyuki
Department Of Internal Medicine Yamaguchi Prefecture General Hospital
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Uehara Masayuki
Division Of Laboratory Animal Research Research Center For Human And Environmental Sciences Shinshu University
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Sugano Mitsutoshi
Department Of Laboratory Medicine Shinshu University Hospital
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MATSUDA Kazuyuki
Department of Laboratory Medicine, Shinshu University Hospital
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- A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min