Plate Assay for Endo-β-N-Acetylglucosaminidase Activity Using a Chromogenic Substrate Synthesized by Transglycosylation with Arthrobacter Endo-β-N-Acetylglucosaminidase

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概要

• 論文の詳細を見る

• The transglycosylation activity of Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A) was used for the enzymatic syntheesis of a novel oligosaccharide, Man_6GlcNac-5-bromo-4-chloro-3-indolyl-β-glucoside (Man_6GlcNAc-Glc-β-X). Various endo-β-N-acetylglucosaminidases hydrolyzed this oligosaccharide, producing Man_6GlcNAc and Glc-β-X. The E. coli strains coexpressing Endo-A and β-glucosidase formed blue colonies in the presence of Man_6GlcNAc-Glc-βX. Therefore, endo-β-N-acetylglucosaminidase activity could be directly detected by the plate assay. This simple plate assay is useful for screening microorganisms for endo-β-N-acetylglucosaminidase activity.

• 社団法人日本生物工学会の論文
• 2000-10-25

著者

• Yamamoto Kenji

  Graduate School of Biostudies, Kyoto University

• TAKEGAWA Kaoru

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Takegawa K

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa K

  Kagawa Univ. Kagawa Jpn

• Yamamoto Kenji

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• Yamamoto K

  Graduate School Of Biostudies Kyoto University

• FUJITA Kiyotaka

  Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University

• Fujita Kiyotaka

  Faculty Of Agriculture Kagoshima University

• Fujita Kiyotaka

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Fujita Kiyotaka

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Asada Yasuhiko

  Department of Bioresource Science, Kagawa University

• Takegawa K

  Dep. Of Life Sciences Fac. Of Agriculture Kagawa Univ.

• Takegawa Kaoru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University:department Of Bios

• Asada Y

  National Institute Of Bioscience And Human-technology Aist Miti

• Asada Yasuhiko

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University

• Asada Yasuo

  National Institute Of Bioscience And Human-technology

• Yamamoto Kenji

  Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University

• Yokozeki Kenzo

  Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University

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