Characterization and Cloning of an Enantioselective Amidase from Comamonas acidovorans KPO-2771-4
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概要
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The characteristics and amino acid sequence of an enantiomer-selective amidase active on R-(-)-2-(3'-benzoyl phenyl)propionamide [R-(-)-ketoprofen amide] purified previously from Coamonas acidivorans KPO-2771-4 were studied. On gel filtration, this amidase appeared to be a monomer with a molecular mass of 55 kDa. It had maximal activity at 35℃ and at pH values from 8.5 to 10.0 Except for Cu^<2+>, Zn^<2+>, Pb^<2+>, and p-chloromercuribenzoate, it was not affected by chelating reagents, carbonyl reagents, reductants, most metal ions, or thiol reagents. The amidase had hydrolyzing activity against a broad range of aliphatic, aromatic, and amino acid amides, evidence that it is a wide-spectrum amidase. Oligonucleotide probes designed from limited peptide sequence information were used to clone the corresponding gene. The nucleotide-determined sequence indicated that the amidase consists of 473 amino acids (M_w 50,464 Da). Significant homologies were found at the amino acid level between the R-enantiomer-selective amidase of C. acidovorans KPO-2771-4 and the S-enantiomer-selective enzymes from Rhodococcus sp., Brevibacterium sp., and Pseudomonas sp. Only 39% homology was conserved in the consensus region of these amidases.
- 社団法人日本生物工学会の論文
- 1997-02-25
著者
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Yamamoto K
Graduate School Of Biostudies Kyoto University
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Otsubo Kazumasa
Technology Development Department Pharmaceutical Production & Technology Center Asahi Chemical I
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HAYASHI TAKAHIKO
Laboratory for Chemistry, Institute for Life Science Research, Asahi Chemical Ind. Co. Ltd.,
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YAMAMOTO KEIZOU
The Noguchi Institute
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MATSUO AKIRA
Technology Development Department, Pharmaceutical Production & Technology Center, Asahi Chemical Ind
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MURAMATSU SHUUJI
Laboratory for Chemistry, Institute for Life Science Research, Asahi Chemical Ind. Co. Ltd.,
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MATSUDA AKIO
Laboratory for Chemistry, Institute for Life Science Research, Asahi Chemical Ind. Co. Ltd.,
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KOMATSU KEN-ICHI
Technology Development Department, Pharmaceutical Production & Technology Center, Asahi Chemical Ind
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Hayashi T
Mukogawa Women's Univ. Nishinomiya Jpn
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Yamamoto K
Department Of Chemistry Nara Medical University
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KOMATSU Ken-ichi
Laboratory for Chemistry, Institute for Life Science Research, Asahi Chemical Industry Co., Ltd.
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Komatsu K
Laboratory For Chemistry Institute For Life Science Research Asahi Chemical Industry Co. Ltd.
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Matsuo Akira
Technology Development Department Pharmaceutical Production & Technology Center Asahi Chemical I
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Muramatsu Shuuji
Laboratory For Chemistry Institute For Life Science Research Asahi Chemical Ind. Co. Ltd.
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