Cloning of cDNAs Encoding Two Peroxidases of Arabidopsis thaliana and Their Organ-Specific Expression
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概要
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Two cDNAs, prxCa and prxEa, encoding peroxidase isozymes of Arabidopsis thaliana, ARP Ca and ARP Ea, respectively, were isolated by polymerase chain reaction using total RNA from stem and primers designed from genomic DNAs. The coding regions of the prxCa and prxEa cDNAs were identical to the putative exon regions in the genomic DNAs (Intapruk, C. et al., Gene, 98,237-241,1991) and contained 1092 and 1078 bp, respectively. The deduced amino acid sequences of ARP Ca and ARP Ea suggested the presence of leader peptides of 31 and 29 amino acid residues at the N-termini, and 308 and 307 amino acid residues in mature proteins, respectively. The amino acid sequence of ARP was compared with those of other plant peroxidases : ARP showed highly conserved alignment with the horseradish peroxidase (HRP) family (group 1), with 64 to 91% homology except for HRP n; lower similarity to rice, tobacco, cucmber, turnip, wheat and peanut peroxidases (group II) with 40 to 51% homology; and a very low level of homology of about 37% for potato and tomato peroxidases. The prxCa and prxEa were close to HRP neutral and basic isozyme genes, respectively, but the estimated isoelectric points of ARP Ca and ARP Ea showed values of 8.05 and 6.34,respectively. By Northern blot hybridization, the prxCa gene was shown to be expressed non-specific expression in many organs of A. thaliana, while the level of transcripts of the prxEa gene was found to be abundant in root but very low in leaf and stem.
- 社団法人日本生物工学会の論文
- 1993-03-25
著者
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Fujiyama Kazuhito
International Center for Biotechnology, Osaka University
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Yamamoto Keiji
Department Of Cardiology Jichi Medical University
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Fujiyama Kazuhito
International Center For Biotechnology Osaka Univ.
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SHINMYO Atsuhiko
Department of Biosciences, Nara Institute of Science and Technology
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Yamamoto K
Graduate School Of Biostudies Kyoto University
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TAKANO MITSUO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Fujiyama Kazuhito
International Center Of Cooperative Research In Biotechnology:faculty Of Engineering Osaka Universit
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INTAPRUK CHOKCHAI
Department of Biotechnology
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Takano M
Institute Of Genetic Ecology Tohoku Universtiy
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Yamamoto K
Department Of Chemistry Nara Medical University
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Intapruk Chokchai
Department Of Biotechnology:faculty Of Engineering Osaka University
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Yamamoto Keiji
Department Of Applied Physics Faculty Of Engineering Tokyo University
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Takano Mitsuo
Department of Biotechnology, Faculty of Engineering, Osaka University
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