Alteration of Catalytic Function of 6-Aminohexanoate-Dimer Hydrolase by Site-Directed Mutagenesis
スポンサーリンク
概要
- 論文の詳細を見る
Alteration of Asp181 in a nylon oligomer-degrading enzyme, 6- aminohexanoate-dimer hydrolase (EII) of Flavobacterium sp. KI72,to Asn and to Glu by site-directed mutagenesis increased K_m values toward 6- aminohexanoate-dimer 4 times and 11 times, respectively. Replacement to His or to Lys caused complete loss of the activity (less than 0.02% of the activity of the EII enzyme). Thus, a single amino acid alteration at position 181 of the enzyme drastically affects the catalytic function.
- 社団法人日本生物工学会の論文
- 1991-03-25
著者
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Fujiyama Kazuhito
International Center for Biotechnology, Osaka University
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Negoro Seiji
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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Negoro Seiji
Department Of Biotechnology Faculty Of Engineering Osaka University
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Negoro Seiji
Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec
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Kazuhito Fujiyama
International Center For Biotechnology Osaka University
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Fujiyama Kazuhito
大阪大学生物工学国際交流センター
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Fujiyama Kazuhito
International Center For Biotechnology Osaka Univ.
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Fujiyama Kazuhito
Department Of Fermentation Technology Osaka University
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Urabe I
Osaka Univ. Osaka Jpn
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Urabe Itaru
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Urabe Itaru
Department Of Biotechnology Faculty Of Engineering Osaka University
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OKADA HIROSUKE
(Present address)Department of Applied Microbial Technology, The Kumamoto Institute of Technology
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HATANAKA HARUYO
Department of Fermentation Technology, Osaka University
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Urabe Itaru
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
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Negoro Seiji
Department Of Biotechnology Osaka University
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Okada H
Kansai Univ. Osaka Jpn
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Okada H
Department Of Fermentation Technology Faculty Of Engineering Osaka University
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Okada H
Nagaoka Univ. Technol. Niigata Jpn
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Okada H
Nagoya City Univ. School Of Medicine Nagoya Jpn
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Okada H
Department Of Fermentation Technology Facutly Of Enginering Osaka Univeristy
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Urabe Itaru
Department Of Biotechnology Osaka University
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Hatanaka Haruyo
Department Of Fermentation Technology Osaka University
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