Structure of Isozyme Genes of Glucose Dehydrogenase from Bacillus megaterium IAM1030

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概要

• 論文の詳細を見る

• Two isozyme genes of glucose dehydrogenase, gdhI and gdhII, were isolated from Bacillus megaterium LAM1030,and their nucleotide sequences were identified. Each gene has an open reading frame of 783 base pairs available to encode a peptide of 261 amino acids. There are two more open reading frames, orf1 and orf2,upstream from ghd1; orfI consists of 684 base pairs acailable to encode a peptide of 228 amino acids, and orf2 consists of 858 base pairs available to encode a peptide of 286 amino acids. The nucleotide sequence of orf2 seemes to be similar to orfX in the gdh operon of B. subtilis. Upstream of orf2,as well as that of orfX, there is a probable promoter sequence very similar to the consensus promoter sequence recognized by B. subtilis RNA polymerase containing the sigma factor σ^G, a new class of sporulation-specific promoters. Downstream from the coding region of orf2,no transcriptional termination signal is found, and soon after orf2,there is a ribo-somal-binding sequence followed by the coding region of gdhI. Therefore, orf2 and gdhI seem to form an operon similar to the orfX-gdh operon of B. subtilis, and be transcribed during sporulation as a polycistronic message. In the upstream regions of gdhII and orf1,on the other hand, there are probable promoter sequences homologous to the postulated consensus promoter sequence recognized by B. subtilis RNA polymerase containing the sigma factor σ^A. Both gdhI and gdhII are expressed in Escherichia coli cells. The phologenic relationships among glucose dehydrogenase genes including gdhI and gdhII are also discussed.

• 社団法人日本生物工学会の論文
• 1990-12-25

著者

• Negoro Seiji

  Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo

• Negoro Seiji

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Negoro Seiji

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Urabe I

  Osaka Univ. Osaka Jpn

• Urabe Itaru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe Itaru

  Department Of Biotechnology Faculty Of Engineering Osaka University

• OKADA HIROSUKE

  (Present address)Department of Applied Microbial Technology, The Kumamoto Institute of Technology

• Urabe Itaru

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Nishimura Tsunehiko

  Department Of Radiology Kyoto Prefectural University Of Medicine

• NAKAI Takako

  Department of Radiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicin

• MAKINO Yasutaka

  Department of Biology, Faculty of Science, Chiba University

• Negoro Seiji

  Department Of Biotechnology Osaka University

• Okada H

  Kansai Univ. Osaka Jpn

• Okada H

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Okada H

  Nagaoka Univ. Technol. Niigata Jpn

• Okada H

  Nagoya City Univ. School Of Medicine Nagoya Jpn

• Okada H

  Department Of Fermentation Technology Facutly Of Enginering Osaka Univeristy

• Urabe Itaru

  Department Of Biotechnology Osaka University

• MITAMURA TOSHIHIDE

  Department of Fermentation Technology, Faculty of Engineering, Osaka University

• EBORA REYNALDO

  (Present adress)Institute of Biotechnology and Applied Microbiology, University of the Philippines a

• Nakai Takako

  Department Of Radiology Graduate School Of Medical Science Kyoto Prefectural University Of Medicine

• Nakai Takako

  Department Of Chemistry For Materials Faculty Of Engineering Mi'e University

• Makino Y

  Osaka Univ. Osaka Jpn

• Ebora Reynaldo

  (present Adress)institute Of Biotechnology And Applied Microbiology University Of The Philippines At

• Makino Yasutaka

  Department Of Biology Faculty Of Science Chiba University

• Mitamura T

  Osaka Univ. Osaka Jpn

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