Construction and Characterization of Phage Libraries Displaying Artificial Proteins with Random Sequences

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概要

• 論文の詳細を見る

• Three phage libraries, PL1, PL2, and PL3, displaying artificial proteins with random sequences were constructed. The artificial proteins, which are model of ancestral proteins, are derivatives of the 25 kinds of random proteins with about 140 amino acid residues produced via random mutagenesis and combinatorial recombination. The random proteins were displayed on the surface of filamentous bacteriophage as fusion protein with the pIII coat protein at an estimated average number on the phage particles in PL1, PL2, and PL3 of 0.32, 0.32, and 0.08, respectively. Each library was shown to express 10^5 to 10^6 kinds of random proteins. With the phage libraries displaying long random peptides, we now have an effective selection system to observe in vitro evolution of new functional proteins from artificial proteins with random sequences.

• 社団法人日本生物工学会の論文
• 2000-09-25

著者

• Yomo Tetsuya

  Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osa

• Yomo T

  Osaka Univ. Osaka Jpn

• Yomo Tetsuya

  Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka

• Urabe I

  Osaka Univ. Osaka Jpn

• Urabe Itaru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe I

  Graduate School Of Engineering Osaka University

• Urabe Itaru

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Yomo Tetsuya

  Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka

• NOZAKI Chikateru

  Research Department 1, The Chemo-Sero-Therapeutic Research Institute

• Urabe Itaru

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• ISHIGURO Naoki

  Department of Orthopaedic Surgery, Nagoya University School of Medicine

• SHIMA YASUFUMI

  Department of Fermentation Technology, Osaka University

• Nakashima Toshihiro

  Department of Physiology, Kyoto Institute of Technorogy

• Yomo Tetsuya

  Osaka University

• Urabe Itaru

  Department Of Biotechnology Osaka University

• YAMAGUCHI MUNEYOSHI

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• YAMAUCHI ASAO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• NOZAKI CHIKATERU

  The Chemo-Sero-Therapeutic Research Institute

• Nakashima Toshihiro

  Department Of Applied Biology Kyoto Institute Of Technology

• Nakaishi Tomoyuki

  Department Of Biotechnology Graduate School Of Engineering Osaka University:the Chemo-sero-therapeut

• Yamauchi Asao

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Shima Yasufumi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Ishiguro Naoki

  Department Of Orthopaedic Surgery And Rheumatology Nagoya University Graduate School Of Medicine

• Ishiguro Naoki

  Department Of Orthopedic Surgery Nagoya University Graduate School Of Medicine

• Ishiguro Naoki

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe Itaru

  Graduate School Of Engineering Osaka University

• Nakashima T

  Department Of Biotechnology Graduate School Of Engineering Osaka University:the Chemo-sero-therapeut

• Yomo Tetsuya

  Erato Jst:graduate School Of Information Science And Technology Osaka University:graduate School Of

• Ichino Naohiro

  Department Of Liver Biliary Tract And Pancreas Diseases Fujita Health University

• Yomo Tetsuya

  Department Of Bioinformatics Engineering Graduate School Of Information Science And Technology Osaka

• Nozaki Chikateru

  Research Department 1 The Chemo-sero-therapeutic Research Institute

• Yamaguchi Muneyoshi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• YAMAGUCHI Muneyoshi

  Department of Applied Microbiology, Forestry and Forest Products Research Institute

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