Effects of Amino Acid Substitution on the Physicochemical Properties of Artificial Proteins with Random Sequences

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概要

• 論文の詳細を見る

• Physicochemical properties of four random proteins, each consisting of about 150 amino acid residues with different sequence identity, were compared to know the correlation between the physicochemical properties and its sequence. The results showed that the extent of the sequence alterations correlated well with the extent of differences in CD spectra, roughly with those in pH-solubility profiles and sedimentation velocity, and not with that in the binding of a hydrophobic fluorescent dye (ANS). Therefore, proteins with similar sequences can have different physicochemical properties, indicating that the extent of mutational effects varies in response to the sequence being altered. This warrants the evolution of a protein in a sequence-specific manner.

• 社団法人日本生物工学会の論文
• 2001-08-25

著者

• Makino Yoshihide

  Department of Biotechnology, Faculty of Engineering (Biotechnology Research Center), Toyama Prefectu

• Yomo Tetsuya

  Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osa

• Yomo Tetsuya

  Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka

• Urabe Itaru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe Itaru

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Yomo Tetsuya

  Department Of Biotechnology Graduate School Of Engineering Osaka University:torest Japan Science And

• YUTANI Katsuhide

  Institute for Protein Research, Osaka University

• Makino Yoshihide

  Department Of Biotechnology Faculty Of Engineering (biotechnology Research Center) Toyama Prefectura

• Makino Yoshihide

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Ogasahara Kyoko

  Institute for Protein Research, Osaka University

• TOKURIKI NOBUHIKO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• SAKAMOTO KAZUHITO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• WALUYO DANANG

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Waluyo Danang

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Ogasahara K

  Inst Protein Res Osaka Univ

• Ogasahara Kyoko

  Institute For Protein Research Osaka University

• Yutani K

  Riken Spring-8 Center Harimia Institute Riken

• Tokuriki Nobuhiko

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Sakamoto Kazuhito

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Yutani Katsuhide

  Riken Spring-8 Center. Harima Inst.

• Urabe Itaru

  Department of Biotechnology, Graduate School of Engineering, Osaka University

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