Cloning, Nucleotide Sequence, and Expression in Escherichia coli of DNA Polymerase Gene (polA) from Thermus thermophilus HB8

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概要

• 論文の詳細を見る

• A gene coding for a thermostable DNA polymerase I (Tth Pol I) was cloned from Thermus thermophilus HB8,and its nucleotide sequence was identified. The Tth Pol I gene (polA) has an open reading frame of 2505 base pairs available to encode a peptide of 834 amino acids. Another incomplete open reading frame was also found upstream from the polA gene. In the deduced amino acid sequence of Tth Pol I, which shows 87% similarity to taht of Thermus aquaticus DNA polymerase, there is the leucine zipper structure from Leu458 to Leu486. The Tth Pol I gene was subcloned in a high-expression vector. Escherichia coli cells harboring the hybrid plasmid produced about 100,000 units of thermostable DNA polymerase in a 200-ml culture.

• 社団法人日本生物工学会の論文
• 1993-10-25

著者

• Asakura Keiko

  Department Of Cardiology Toyohashi Heart Center

• Yomo Tetsuya

  Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osa

• Yomo T

  Osaka Univ. Osaka Jpn

• Yomo Tetsuya

  Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka

• Urabe I

  Osaka Univ. Osaka Jpn

• Urabe Itaru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Urabe I

  Graduate School Of Engineering Osaka University

• Urabe Itaru

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Yomo Tetsuya

  Department Of Bioinformatic Engineering Graduate School Of Information Science And Technology Osaka

• Yomo Tetsuya

  Department Of Biotechnology Graduate School Of Engineering Osaka University:torest Japan Science And

• Urabe Itaru

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Asakura Keiko

  Department Of Biotechnology Faculty Of Engineering Osaka University

• KOMATSUBARA HIDEYUKI

  Tsuruga Institute of Biotechnology, Toyobo Co. Ltd.

• OKA MASANORI

  Tsuruga Institute of Biotechnology, Toyobo Co. Ltd.

• Urabe Itaru

  Department Of Biotechnology Osaka University

• KOMATSUBARA HIDEYUKI

  Toyobo Tsuruga Institute of Biotechnology

• SOGA SHIRO

  Department of Biotechnology, Faculty of Engineering, Osaka University

• OKA MASANORI

  Toyobo Tsuruga Institute of Biotechnology

• EMI SHIGENORI

  Toyobu Tsuruga Institute of Biotechnology

• Soga Shiro

  Tokyo Research Laboratories Kyowa Hakko Co. Ltd.

• Soga Shiro

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Emi Shigenori

  Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.

• Urabe Itaru

  Graduate School Of Engineering Osaka University

• Yomo Tetsuya

  Department Of Bioinformatics Engineering Graduate School Of Information Science And Technology Osaka

• Komatsubara Hideyuki

  Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.

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