Mutational Analysis of the Metabolism of 2,6-Naphthalenedisulfonate by Pigmentiphaga sp. NDS-2

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概要

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• The metabolism of 2,6-naphthaIenedisulfonate (26NDS) by Pigmentiphaga sp. NDS-2 was analyzed by isolating a mutant (NDS2-002) which slowly grew on a minimal medium containing 26NDS as the sole source of carbon. Liquid chromatography/mass spectrometry (LC/MS) analysis of metabolic intermediates in this strain revealed that 5-sulfosalicylate (5SSA) is accumulated during the degradation of 26NDS. To analyze the lower metabolic pathway, a mutant strain NDS2-008, which could not grow either on 26NDS, 5SSA or gentisate, but could on succinate as the carbon source, was isolated. When the resting cells of NDS2-008 were incubated with 5SSA or gentisate, a substance deduced to be maleylpyruvate (Zhou et al, J. Bacteriol., 183, 700-708, 2001) was commonly detected upon HPLC analysis. These results suggest that Pigmentiphaga sp. NDS-2 degrades 26NDS via 5SSA, gentisate and maleylpyruvate as intermediates.

• 2003-05-25

著者

• Takeo Masahiro

  Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo

• Negoro Seiji

  Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo

• UCHIHASHI KINYA

  Department of Materials Science and Chemistry, Graduate School of Engineering, Himeji Institute of T

• MISAWA TAKUMA

  Department of Materials Science and Chemistry, Graduate School of Engineering, Himeji Institute of T

• Negoro Seiji

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Negoro Seiji

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Misawa Takuma

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Negoro S

  Himeji Inst. Technol. Himeji Jpn

• Takeo M

  Himeji Inst. Technol. Hyogo Jpn

• Takeo Masahiro

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

• Takeo Masahiro

  Dept.of Applied Chemistry Himeji Institute Of Technology

• Takeo Masahiro

  Department Of Applied Chemistry Himeji Institute Of Technology

• Takeo Masahiro

  Department Of Applied Chemistry Himeiji Institute Of Technology

• Negoro Seiji

  Department Of Biotechnology Osaka University

• Uchihashi Kinya

  Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec

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