Trichloroethylene Degradation by Genetically Engineered Bacteria Carrying Cloned Phenol Catabolic Genes

概要

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Pseudomonas putida BH was capable of degrading trichloroethylene (TCE) when grown on phenol, o-crsol, m-cresol or p-cresol, each of which induced the enzymes catalyzing the catechol catabolism through the meta-cleavage pathway. Various DNA fragments containing phenol/cresol catabolic genes cloned from this strain were introduced into Escherichia coli and P. putida strains by using plasmid vectors, and the resultant recombinants were examined for their TCE-degrading ability. The recombinants harboring and expressing the phenol hydroxylase gene exhibited an ability to degrade TCE in phosphate buffer, while the rates and extents of TCE degradation depended considerably on the host strain and copresence of other phenol catabolic genes. During TCE degradation by BH and recombinant E. coli, nearly complete dechlorination occurred. A recombinant E. coli strain did not require phenol as a cosubstrate for TCE degradation, the rate of which was comparable to that of fully-induced P. putida BH. It did, however, require isopropyl-β-D-thiogalactopyranoside for induction of the lac promoter on the vector.
社団法人日本生物工学会の論文
1995-02-25