Potential of Predominant Activated Sludge Bacteria as Recipients in Conjugative Plasmid Transfer(ENVIRONMENTAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
We investigated the possibility of conjugative plasmid transfer to the predominant bacteria in activated sludge and the factors influencing the transfer frequency in the activated sludge process. We performed conjugative transfers of a self-transmissible, broad-host-range plasmid RP4 from Escherichia coli C600 to activated sludge bacteria by broth mating. Most of the activated sludge bacteria tested could acquire plasmid RP4, although the transfer frequencies varied from 8.8×10^<-7> to 1.3×10^<-2> transconjugants per recipient. The transfer frequencies in several strains were similar to, or higher than, that in intraspecific transfer to E. coli HB101. Matings under various environmental conditions showed that factors relevant to physiological activity, such as temperature and nutrient conditions, seemed to affect the transfer frequency. In addition, conjugative transfer was detected even in filtered raw and treated wastewaters. Thus, the predominant activated sludge bacteria seem to have sufficient potential as recipients in conjugative plasmid transfer under the conditions likely to occur in the activated sludge process. Transfer frequency was reduced by agitation in the presence of suspended solid. This may suggest that conjugative plasmid transfer is physically inhibited in aeration tanks.
- 社団法人日本生物工学会の論文
- 2005-12-25
著者
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Ike Michihiko
Division of Sustainable Energy and Environmental Engineering, Osaka University
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SODA Satoshi
Division of Endocrinology and Metabolism, Niigata University Graduate School of Medical and Dental S
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Inoue Daisuke
Division of Sustainable Energy and Environmental Engineering, Osaka University
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Sei Kazunari
Division of Sustainable Energy and Environmental Engineering, Osaka University
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Fujita Masanori
Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka
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Ike M
Division Of Sustainable Energy And Environmental Engineering Osaka University
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Fujita M
Kochi National College Of Technology
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Inoue Daisuke
Division Of Sustainable Energy And Environmental Engineering Graduate School Of Engineering Osaka Un
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Sei Kazunari
Dept. Mat. Env. Energy. Osaka Univ.
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Fujita Masanori
Kochi National College of Technology
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Fujita Masanori
Environ. Eng. Osaka Univ.
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Fujita Masanori
Graduate School Of Engineering Osaka University
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Ike Michihiko
Division Of Sustainable Energy And Environmental Engineering Osaka University
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Ike Michihiko
大阪大学 環境・エネルギー工学専攻
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Ike Michihiko
Division Of Sustainable Energy And Environmental Engineering Graduate School Of Engineering Osaka Un
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Inoue Daisuke
Division Of Endocrinology And Metabolism Department Of Internal Medicine Hyogo Prefectural Amagasaki
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Soda S
National Institute For Environmental Studies
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Soda Satoshi
Division Of Endocrinology And Metabolism Niigata University Graduate School Of Medical And Dental Sc
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Ike Michihiko
Environ. Eng. Osaka Univ.
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Fujita Masakatsu
Kochi National College Of Technology
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Fujita M
Department Of Environmental Engineering Osaka University
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Ike M
Osaka University
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Ike Michihiko
Department Of Environmental Engineering Faculty Of Engineering Osaka University
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Sei K
Dept. Mat. Env. Energy. Osaka Univ.
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Sei Kazunari
Division Of Sustainable Energy And Environmental Engineering Graduate School Of Engineering Osaka Un
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Ike M
Division Of Sustainable Energy And Environmental Engineering Graduate School Of Engineering Osaka Un
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Fujita M
Graduate School Of Biosphere Science Hiroshima University
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Fujita Masanori
Division Of Sustainable Energy And Environmental Engineering Graduate School Of Engineering Osaka Un
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Inoue Daisuke
Division Of Artificial Environment Systems Yokohama National University
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Inoue Daisuke
Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University
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SODA SATOSHI
Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University
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FUJITA Masanori
Division of Environmental Medicine, National Defense Medical College Research Institute
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