Characterization of a Bioflocculant Produced by Citrobacter sp. TKF04 from Acetic and Propionic Acids

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概要

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• A bacterial strain, TKF04, capable of producing a bioflocculant from acetic and/or propionic acids was isolated from a biofilm formed in inside a kitchen drain. It was identified as a Citrobacter based on its morphological and physiological characteristics and the partial sequences of its 16S rRNA. TKF04 produced the bioflocculant during the logarithmic phase of growth, and the optimum temperature and pH for the bioflocculant production were 30℃ and 7.2-10.0, respectively. It could utilize some organic acids and sugars for its growth as the sole carbon sources when yeast extract was supplemented; however, only acetate and propionate were found to be good substrates for the bioflocculant production. The crude bioflocculant could be recovered from the supernatant of the culture broth by ethanol precipitation and dialysis against deionized water. It was found to be effective for flocculation of a kaolin suspension, when added at a final concentration of 1-10 mg/l, over a wide range of pHs (2-8) and temperatures (approximately 3-95℃), while the co-presence of cations (Na^+, K^+, Ca^<2+> Mg^<2+> Fe^<2+> Al^<3+> or Fe^<3+>) did not enhance the flocculating activity. It could efficiently flocculate a variety of inorganic and organic suspended particles, including kaolin, diatomite, bentonite, activated carbon, soil and activated sludge. It contained glucosamine as the major component, and the molecular weight was estimated to be between 232 and 440 kDa by gel filtration. The observation that the flocculating activity was completely lost following chitinase treatment and its analysis with a Fourier transform infrared spectrometer suggested that the bioflocculant is a biopolymer structurally - similar to chitin or chitosan.

• 社団法人日本生物工学会の論文
• 2000-01-25

著者

• Ike Michihiko

  Department Of Environmental Engineering Graduate School Of Engineering Osaka University

• Kim S

  Osaka Univ. Osaka Jpn

• Kim Shin

  Department Of Orthopedics Kyungpook National University

• FUJITA Masanori

  Department of Environmental Engineering, Osaka University

• Fujita M

  Kochi National College Of Technology

• Fujita Masanori

  Kochi National College of Technology

• Fujita Masanori

  Environ. Eng. Osaka Univ.

• Kim Shin

  Department Of Computer Science Yousei University

• Ike Michihiko

  Environ. Eng. Osaka Univ.

• Fujita Masakatsu

  Kochi National College Of Technology

• TACHIBANA Shinya

  Department of Environmental Engineering, Graduate School of Engineering, Osaka University

• KITADA Go

  Department of Environmental Engineering, Graduate School of Engineering, Osaka University

• INOUE ZENSUKE

  Research Center, Takuma Co. Ltd.

• Fujita M

  Department Of Environmental Engineering Osaka University

• Ike M

  Osaka University

• Ike Michihiko

  Department Of Environmental Engineering Faculty Of Engineering Osaka University

• Inoue Z

  Research Center Takuma Co. Ltd.

• Kitada Go

  Department Of Environmental Engineering Graduate School Of Engineering Osaka University

• Ike M

  Division Of Sustainable Energy And Environmental Engineering Graduate School Of Engineering Osaka Un

• Ike M

  Osaka Univ.

• Tachibana Shinya

  Department Of Environmental Engineering Graduate School Of Engineering Osaka University

• Fujita M

  Graduate School Of Biosphere Science Hiroshima University

• Fujita Masanori

  Department Of Environmental Engineering Faculty Of Engineering Osaka University

• Fujita Masanori

  Department Of Cardiology Saiseikai Izuo Hospital

• Kim Shin

  Department Of Clinical Immunology Aids Research And Surgery Institute Of Medical Science University

• Fujita Masanori

  Department Of Animal Science Faculty Of Agriculture Tohoku University:(present Address) Department O

• Fujuta Masanori

  Department of Environmental Engineering, Osaka University

• Shinya Tazhibane

  Department of Environmental Engineering, Osaka University

• Tathibana Shinya

  Department of Environmental Engineering, Osaka University

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