Analysis of Pellet Formation of Aspergillus niger Based on Shear Stress

概要

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A theoretical discussion on pellet formation of Aspergillus niger based on shear rate or averge velocity gradient (G value) was made for both cases of a reactor with paddle and a rotationally shaken flask. The average diameter of pellets (d) and the number of pellets formed per unit volume (n) are linearly to the G value below 400 1/s on a logarithmic plot, and a regression equaton, n=(1.37×10^<-5>)・d^<-3.25>, was obtained regardless of the agitation and inoculation conditions. Under non-turbulent agitaiton conditions, the average number of spores forming a single pellet was found to be approximately proportional to the two-thirds power of the concentration of the spore inculated. These exponents are very close to the theoretical one. Consequently it is concluded that G value is a representative index for the evaluation of pellent formation capability, verifying the validity of the proposed theory.
社団法人日本生物工学会の論文
1994-11-25