Application of a Floc-Forming Genetically Engineered Microorganism to a Sequencing Batch Reactor for Phenolic Wastewater Treatment

概要

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For enhancing the survival of genetically engineered microorganisms(GEMs)in activated sludge processes, the use of a floc-forming bacterium as the host for a recombinant plasmid was proposed. The floc-forming and phenol-degrading GEM Sphingomonas paucimobilis 551(pS10-45)was cultured to demonstrate this proposal. Although the maximum growth rate of the host strain S.paucimobilis 551 was low and the recombinant plasmid pS10-45 was unstable in the host, the resultant GEM S.paucimobilis 551(pS10-45)was difficult to wash out together with the effluent, and it maintained population 3-4 times higher than the non-floc-forming GEM Escherichia coli HB101(pS10-45)in a model activated sludge process operated in a sequencing batch mode. In the long run, the GEM-inoculated activated sludge process showed better phenol removal ability by the recombinant plasmid and better sludge settlement by the host strain.
社団法人日本生物工学会の論文
1999-07-25