Enzymatic Degradation of Cellulose Acetate Plastic by Novel Degrading Bacterium Bacillus sp. S2055

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概要

• 論文の詳細を見る

• Cellulose acetate (CA)-degradingbacteria were isolated from samples obtained from environments at a population size of 6.7×10^1 to 1.0×10^8 halo-forming cfu/ml-water or g-solid, suggesting their ubiquitous presence. The classification of 35 isolated strains of CA-degrading bacteria into 15 genera indicates that CA-degrading activity is over a wide range of taxonomical groups. From these isolates, Bacillus sp.S2055 was found to be the most efficient CA-degrading bacterium, and its CA-degrading enzyme(s) was partially characterized. The weight loss of CA plastic film (degree of substitution (DS)=l7)in the culture of S2055 was less than 12% after a 35-d culture while that in the crude enzyme solution extracted from the culture supernatant reached 62% after the same period. Lipase and cellulase activities were detected in the culture supernatant of strain S2055. The crude enzyme solution contained three major protein fractions that have differentr mean molecular weights (MWs). Fraction I with the highest MW exhibited both lipase and cellulase activities, while fraction II and III exhibited only lipase activity. Fraction I significantly deacetylated CA (DS 1.5) and fragmented CA plastic film into pleces while the other fractions failed to do so even when used in combination with commercially-available cellulases and lipases. The lipase activity of fraction I against various substrates differed considerably from those of known lipases. It was thus suggested that deacetylation of CA mediated by an enzyme with such a peculiar lipase-like activity is a requisite for the efficient biodegradation of CA plastics.

• 社団法人日本生物工学会の論文
• 2000-10-25

著者

• Ishigaki Tomonori

  Department of Environment Solution Technology, Faculty of Science and Technology, Ryukoku University

• Ike Michihiko

  Department Of Environmental Engineering Graduate School Of Engineering Osaka University

• FUJITA Masanori

  Department of Environmental Engineering, Osaka University

• Fujita M

  Kochi National College Of Technology

• Fujita Masanori

  Kochi National College of Technology

• Fujita Masanori

  Environ. Eng. Osaka Univ.

• Fujita Masanori

  Graduate School Of Engineering Osaka University

• Ike Michihiko

  Division Of Sustainable Energy And Environmental Engineering Osaka University

• Ike Michihiko

  大阪大学 環境･エネルギー工学専攻

• Ike Michihiko

  Environ. Eng. Osaka Univ.

• Fujita Masanori

  Kochi National Coll. Of Technol. 200-1 Monobe Otsu Nankoku Kochi 783-8508 Jpn

• SUGANO WATARU

  Department of Environmental Engineering, Graduate School of Engineering, Osaka University

• Fujita M

  Department Of Environmental Engineering Osaka University

• Ike M

  Osaka University

• Ike Michihiko

  Department Of Environmental Engineering Faculty Of Engineering Osaka University

• Sugano Wataru

  Department Of Environmental Engineering Graduate School Of Engineering Osaka University

• Ike M

  Division Of Sustainable Energy And Environmental Engineering Graduate School Of Engineering Osaka Un

• Ishigaki Tomonori

  Department Of Environment Solution Technology Faculty Of Science And Technology Ryukoku University

• Fujita M

  Graduate School Of Biosphere Science Hiroshima University

• Fujita Masanori

  Department Of Environmental Engineering Faculty Of Engineering Osaka University

• Fujita Masanori

  Department Of Cardiology Saiseikai Izuo Hospital

• Fujita Masanori

  Department Of Animal Science Faculty Of Agriculture Tohoku University:(present Address) Department O

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