Molecular Cloning and Sequencing of the Phenol Hydroxylase Gene from Pseudomonas putida BH
スポンサーリンク
概要
- 論文の詳細を見る
A genomic library of the phenol-degrading bacterium Pseudomonas putida BH was constructed in the broad host range cosmid pVK100 and introduced into Escherichia coli HB101. One of the recombinant cosmids recovered from catechol- and/or 2-hydroxymuconic semialdehyde-accumulating clones, pS10-45,had a 19.6-kb insert fragment which allowed P. putida KT2440 to grow on phenol as a sole carbon and energy source. Subcloning and expression studies indicated that the phenol hydroxylase gene cluster (pheA) is located on a 6.1-kb SacI fragment. The results of DNA sequencing of the SacI fragment revealed that the pheA gene cluster encodes a multicomponent phenol hydroxylase.
- 社団法人日本生物工学会の論文
- 1995-05-25
著者
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Takeo Masahiro
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo
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MORI Kazuhiro
Department of Pediatrics, School of Medicine, University of Tokushima
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OKADA HIROSHI
Department of Urology, Kobe University School of Medicine
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Ike Michihiko
Department Of Environmental Engineering Graduate School Of Engineering Osaka University
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Okada Hiroshi
Department Of Urology For Medical Research Kobe University School Of Medicine
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Mori K
Department Of Civil And Environmental Engineering University Of Yamanashi
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Takeo M
Himeji Inst. Technol. Hyogo Jpn
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Takeo Masahiro
Department Of Materials Science And Chemistry Graduate School Of Engineering Himeji Institute Of Tec
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Takeo Masahiro
Dept.of Applied Chemistry Himeji Institute Of Technology
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Takeo Masahiro
Department Of Applied Chemistry Himeji Institute Of Technology
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Takeo Masahiro
Department Of Applied Chemistry Himeiji Institute Of Technology
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FUJITA Masanori
Department of Environmental Engineering, Osaka University
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MAEDA YOSHIMICHI
Department of Applied Chemistry, Himeji Institute of Technology
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MIYAMA KANAKO
Department of Environmental Engineering, Osaka University
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Miyama Kanako
Department Of Environmental Engineering Osaka University
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Fujita M
Kochi National College Of Technology
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Fujita M
Osaka Univ. Osaka Jpn
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Maeda Yoshimichi
Dept.of Applied Chemistry Himeji Institute Of Technology
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Maeda Yoshimichi
Department Of Applied Chemistry Faculty Of Engineering Himeji Institute Of Technology
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Mori K
College Of Liberal Arts Toyama University
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Mori K
Faculty Of Engineering Toyama University
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Mori Katunori
Department Of Physics Faculty Of Science Toyama University
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Fujita Masanori
Kochi National College of Technology
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Fujita Masanori
Environ. Eng. Osaka Univ.
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Fujita M
Department Of Environmental Engineering Faculty Of Engineering Osaka University
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Mori K
Institute Of Materials Structure Science High Energy Accelerator Research Organization
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Ike Michihiko
Division Of Sustainable Energy And Environmental Engineering Osaka University
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Ike Michihiko
Environ. Eng. Osaka Univ.
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Fujita Masanori
Kochi National Coll. Of Technol. 200-1 Monobe Otsu Nankoku Kochi 783-8508 Jpn
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Fujita M
Department Of Environmental Engineering Osaka University
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Maeda Y
Himeji Inst. Technol. Hyogo Jpn
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Okada H
Botanical Gardens Faculty Of Science Osaka City University
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Ike Michihiko
Department Of Environmental Engineering Faculty Of Engineering Osaka University
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Mori Kazuhiro
Department Of Anatomy Okayama University Medical School:(present Address) Section Of Pathology Osaka
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Okada Hiroshi
Department Of Anesthesiology Iwate Medical University School Of Medicine
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Fujita Masanori
Department Of Environmental Engineering Faculty Of Engineering Osaka University
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Fujita Masanori
Department Of Cardiology Saiseikai Izuo Hospital
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Fujita Masanori
Department Of Animal Science Faculty Of Agriculture Tohoku University:(present Address) Department O
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Mori Kazuhiro
Department of Civil and Environmental Engineering, University of Yamanashi
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