Development and Application of PCR Primers for Monitoring Alkane-Degrading Bacteria in Seawater Microcosm during Crude Oil Degradation Process
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概要
- 論文の詳細を見る
For extensive detection and monitoring of alkane-degrading bacteria, three combinations of PCR primer sets and gene probes were designed based on the homologous regions within a variety of alkane hydroxylase genes (alk genes) registered in the GenBank and examined their availability. PCR using the designed primers amplified DNA fragments with the expected sizes from all the tested bacterial strains used for primer design, and all of the amplified fragments gave positive results by Southern hybridization with the designed probes. The primers amplified DNA fragments with expected sizes from all the 76 wild-type newly isolated strains, while the probes gave positive results against the amplified fragments from 60 strains. Thus, the primer/probe system established in the present study can detect most of the alkane-degrading bacteria, and can be applied to evaluate alkane-degradation potential in the environments.Applying the designed primers, behavior of microbial populations responsible for the degradation of n-alkanes, a major component of crude oil, was monitored in seawater microcosm during the crude oil degradation process to clarify the degradation mechanisms of n-alkanes. It revealed that the alkane-degrading bacteria altered according to the fates of n-alkanes with different carbon chain lengths, so that shorter alkanes were degraded first by alkane-degrading bacteria possessing alk genes of Group I (Group I alkane-degrading bacteria) and longer ones afterwards by Group II alkane-degrading bacteria.
- 社団法人 化学工学会の論文
- 2003-10-01
著者
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MORI Kazuhiro
Department of Pediatrics, School of Medicine, University of Tokushima
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Mori K
Department Of Civil And Environmental Engineering University Of Yamanashi
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SEI KAZUNARI
Department of Civil and Environmental Engineering, University of Yamanashi
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KOHNO TETSURO
Department of Civil and Environmental Engineering, University of Yamanashi
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MAKI HIDEAKI
National Institute for Environmental Studies
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Kohno Tetsuro
Department Of Civil And Environmental Engineering University Of Yamanashi
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Sei Kazunari
Dept. Mat. Env. Energy. Osaka Univ.
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Mori K
College Of Liberal Arts Toyama University
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Mori K
Faculty Of Engineering Toyama University
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Mori Katunori
Department Of Physics Faculty Of Science Toyama University
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Mori K
Institute Of Materials Structure Science High Energy Accelerator Research Organization
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Sei K
Dept. Mat. Env. Energy. Osaka Univ.
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Mori Kazuhiro
Department Of Anatomy Okayama University Medical School:(present Address) Section Of Pathology Osaka
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Mori Kazuhiro
Department of Civil and Environmental Engineering, University of Yamanashi
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