Diversity and Similarity of Microbial Communities in Petroleum Crude Oils Produced in Asia

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概要

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• To understand microbial communities in petroleum crude oils, we precipitated DNA using high concentrations of 2,2,4-trimethylpentane (isooctane) and purified. Samples of DNA from five crude oils, (Middle East, 3; China, 1; and Japan, 1) were characterized based upon their 16S rRNA gene sequences after PCR amplification and the construction of clone libraries. We detected 48 eubacterial species, one cyanobacterium, and one archaeon in total. The microbial constituents were diverse in the DNA samples. Most of the bacteria affiliated with the sequences of the three oils from the Middle East comprised similar mesophilic species. Acinetobacter, Propionibacterium, Sphingobium and a Bacillales were common. In contrast, the bacterial communities in Japanese and Chinese samples were unique. Thermophilic Petrotoga-like bacteria (11%) and several anaerobic-thermophilic Clostridia- and Synergistetes-like bacteria (20%) were detected in the Chinese sample. Different thermophiles (12%) and Clostridia (2%) were detected in the Japanese sample.

• 社団法人 日本農芸化学会の論文

著者

• Kitaoka Motomitsu

  National Food Research Institute, National Agriculture and Food Research Organization

• Uchiyama Hiroo

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Nomura Nobuhiko

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• MAKI HIDEAKI

  National Institute for Environmental Studies

• Yamane Kunio

  National Food Research Institute

• Nakayama Tsuyoshi

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• NAKAJIMA Toshiaki

  Graduate School of Life and Environmental Sciences, University of Tsukuba

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