Syntheses of 4-Methylumbelliferyl-β-D-Xylobioside and 5-Bromo-3-Indolyl-β-D-Xylobioside for Sensitive Detection of Xylanase Activity on Agar Plates

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概要

• 論文の詳細を見る

• 4-Methylumbelliferyl-β-D-xylobioside (MU-X_2) and 5-bromo-3-indolyl-β-D-xylobioside (BI-X_2) were synthesized as substrates for the detection of xylanase activity on agar plates. A family F/10 xylanase from Steptomyces olivaceoviridis E-86 (FXYN) was able to be more sensitively detected than RBB-xylan by using MUX_2 as a substrate. A mutant xylanase E128H/FXYN having only 1/1000 of the activity of FXYN was also able to be detected on the MU-X_2 plate but was not detected on the RBB-xylan plate. A family G/11 xylanase from Streptomyces lividans 66 (Xyn B) was not detected on the MU-X_2 plate, but it was able to be detected on the RBB-xylan plate, suggesting that the MU-X_2 substrate is specific to family F/10 xylanases. However, none of the xylanases were detected effectively by using BI-X_2 as a substrate.

• 社団法人日本農芸化学会の論文
• 2000-04-23

著者

• Kitaoka Motomitsu

  National Food Research Institute, National Agriculture and Food Research Organization

• Kobayashi H

  National Food Research Institute

• KANEKO Satoshi

  Food Biotechnology Division, National Food Research Institute

• Kaneko Satoshi

  Food Biotechnology Div. National Food Res. Inst.

• Kuno Atsushi

  Research Center For Medical Glycoscience National Institute Of Advanced Industrial Science And Techn

• Kuno Atsushi

  Department Of Obstetrics And Gynecology Suzuki Hospital

• KANEKO Satoshi

  National Food Research Institute

• Kobayashi H

  National Food Res. Inst. Ibaraki Jpn

• Kitaoka Motomitsu

  National Food Res. Inst.

• Kuno Atsushi

  Department Of 2nd Internal Medicine Sapporo Medical University School Of Medicine

• Kiuchi Hiroshi

  Research Development Section Kibun Food Chemifa Co. Ltd.

• Hayashi Kiyoshi

  National Food Research Institute Ministry Of Agriculture Forestry And Fisheries

• Hayashi K

  National Food Research Institute Ministry Of Agriculture Forestry And Fisheries

• Kaneko Satoshi

  National Food Res. Inst.

• Kaneko S

  Food Biotechnology Division National Food Research Institute

• Kaneko Shigenobu

  National Institute Of Crop Science (nics)

• Kitaoka Motomitsu

  Nationalfood Research Institute Ministry Of Agriculture Forestry And Fisheries

• Hayashi K

  Department Of Biochemical Sciences Kagoshima University

• Kaneko Satoshi

  Food Biotechnology Division National Food Research Institute

• Hayashi Kiyoshi

  National Food Research Institute

• Kitaoka Motomitsu

  National Agriculture And Food Research Organization National Food Research Institute

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