The Tetramer Structure of the Glycoside Hydrolase Family 27 α-Galactosidase I from Umbelopsis vinacea
スポンサーリンク
概要
- 論文の詳細を見る
The crystal structure of Umbelopsis vinacea α-galactosidase I, which belongs to glycoside hydrolase family 27, was determined at 2.0 Å resolution. The monomer structure was well conserved with those of glycoside hydrolase family 27 enzymes. The biological tetramer structure of this enzyme was constructed by the crystallographic 4-fold symmetry, and tetramerization appeared to be caused by three inserted peptides that were involved in the tetramer interface. The quaternary structure indicated that the substrate specificity of this enzyme might be related to the tetramer formation. Three N-glycosylated sugar chains were observed, and their structures were found to be of the high-mannose type.
著者
-
KANEKO Satoshi
Food Biotechnology Division, National Food Research Institute
-
FUJIMOTO Zui
Protein Research Unit, National Institute of Agrobiological Sciences
-
Kim Wook-Dong
Food Biotechnology Division, National Food Research Institute
-
PARK Gwi-Gun
Food Biotechnology Division, National Food Research Institute
-
MOMMA Mitsuru
Protein Research Unit, National Institute of Agrobiological Sciences
-
KOBAYASHI Hideyuki
Food Biotechnology Division, National Food Research Institute
-
Kaneko Satoshi
Food Biotechnology Div. National Food Res. Inst.
-
Kaneko Satoshi
National Food Res. Inst.
関連論文
- Molecular cloning of cDNAs encoding two glycoside hydrolase family 7 cellobiohydrolases from the basidiomycete Flammulina velutipes
- Crystallographic Snapshots of an Entire Reaction Cycle for a Retaining Xylanase from Streptomyces olivaceoviridis E-86
- The Tetramer Structure of the Glycoside Hydrolase Family 27 α-Galactosidase I from Umbelopsis vinacea
- Characterization of α-L-arabinofuranosidase related to the secondary cell walls formation in Arabidopsis thaliana
- Cadmium Concentration in Grains of Japanese Wheat Cultivars : Genotypic Difference and Relationship with Agronomic Characteristics(Genetic Resources Evaluation)
- Uzu Mutation in Barley (Hordeum vulgare L.) Reduces the Leaf Unrolling Response to Brassinolide(Organic Chemistry)
- 1P-023 芳香環水酸化ジオキシゲナーゼの電子伝達コンポーネント(ferredoxin reductase)のX線結晶構造解析(蛋白質・構造(1),第46回日本生物物理学会年会)
- Screening for Phenoloxidases from Edible Mushrooms(Biochemistry & Molecular Biology)
- Purification and Characterization of an α-L-Arabinofuranosidase from suspension cultured Non-embryo genic carrot (Daucus carota. L)
- PCR Cloning and Expression of the F/10 Family Xylanase Gene from Streptomyces olivaceoviridis E-86
- Substrate Specificity of the α-L-Arabinofuranosidase from Trichoderma reesei
- Substrate Specificites of α-L-Arabinofuranosidases Produced by Two Species of Aspergillus niger
- ISOLATION AND CHARACTERIZATION OF BORATE-R HAMNOGALACTURONAN-I I COMPLEX FROM SOLUBLE FRACTION OF RADISH ROOTS
- Syntheses of 4-Methylumbelliferyl-β-D-Xylobioside and 5-Bromo-3-Indolyl-β-D-Xylobioside for Sensitive Detection of Xylanase Activity on Agar Plates
- Purification and Characterization of a Family G/11 β-Xylanase from Streptomyces olivaceoviridis E-86
- Characterization of Glycoside Hydrolase Family 6 Enzymes from Coprinopsis cinerea
- Expression of Rice (Oryza sativa L. var. Nipponbare) α-Galactosidase Genes in Escherichia coli and Characterization
- Characterization of a Thermostable Endo-β-1,4-D-galactanase from the Hyperthermophile Thermotoga maritima
- Synergy Between an α-L-Arabinofuranosidase from Aspergillus oryzae and an Endo-Arabinanase from Streptomyces coelicolor for Degradation of Arabinan
- Expression of Streptomyces coelicolor α-Galactosidase Gene in Escherichia coli and Characterization
- Purification, Characterization, and cDNA Cloning of a Novel α-Galactosidase from Mortierella vinacea
- Purification and Characterization of Recombinant Mortierella vinacea α-Galactosidases I and II Expressed in Saccharomyces cerevisiae
- Direct Detection for β-Xylanase on Isoelectric Focusing Gels by Using 2,3,5-Triphenyltetrazolium Chloride
- Detection of α-L-Arabinofuranosidase Activity in Isoelectric Focused Gels using 6-Bromo-2-naphthyl-α-L-arabinofuranoside
- Cloning, Sequencing and Expression of the Cellobiose Phosphorylase Gene of Cellvibrio gilvus
- Cloning and Transcript Analysis of Multiple Genes Encoding the Glycoside Hydrolase Family 6 Enzyme from Coprinopsis cinerea
- Chitosanase-Catalyzed Hydrolysis of 4-Methylumbelliferyl β-Chitotrioside
- Purification and Characterization of Extracellular β-Galactosidase Secreted by Supension Cultured Rice (Oryza sativa L.) Cells(Biochemistry & Molecular Biology)
- Characterization of an Exo-β-1,3-D-galactanase from Streptomyces avermitilis NBRC14893 Acting on Arabinogalactan-Proteins
- Expression of Rice (Oryza sativa L. var. Nipponbare) α-Galactosidase Genes in Escherichia coli and Characterization
- Characterization of Glycoside Hydrolase Family 6 Enzymes from Coprinopsis cinerea
- Development of a Gene Transfer System for the Mycelia of Flammulina velutipes Fv-1 Strain
- Properties of Ethanol Fermentation by Flammulina velutipes
- Use of Whole Crop Sorghums as a Raw Material in Consolidated Bioprocessing Bioethanol Production Using Flammulina velutipes
- Analysis of Calcium and Magnesium Ions in Wheat Flour by Capillary Zone Electrophoresis
- Molecular Cloning and Expression in Pichia pastoris of a Irpex lacteus Exo-β-(1→3)-galactanase Gene
- The Tetramer Structure of the Glycoside Hydrolase Family 27 α-Galactosidase I from Umbelopsis vinacea
- Improvement of the Transformation Efficiency of Flammulina velutipes Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter
- Cloning and Transcript Analysis of Multiple Genes Encoding the Glycoside Hydrolase Family 6 Enzyme from Coprinopsis cinerea
- Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis of Miscanthus sinensis to Monosaccharides
- Long Terminal Repeat of the Mushroom Retrotransposon Functions as a Dual Terminator to Transcription
- Distribution of Hydrophobin 1 Gene Transcript in Developing Fruiting Bodies of Lentinula edodes(Biochemistry & Molecular Biology)
- A Single Amino Acid Substitution Enhances the Catalytic Activity of Family 11 Xylanase at Alkaline pH
- Catalytic Activity of Tripeptidase from Lactococcus lactis to Which Amino Acid Substitution Was Introduced According to Natural Mutation
- Extracellular Carbohydrate Esterase from the Basidiomycete Coprinopsis cinerea Released Ferulic and Acetic Acids from Xylan
- Effect of Lime Pretreatment of Brown Midrib Sorghums