Purification and Characterization of a Family G/11 β-Xylanase from Streptomyces olivaceoviridis E-86
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概要
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A β-xylanase (GXYN) was purified from the culture filtrate of Streptomyces olivaceoviridis E-86 by successive chromatography on DE-52,CM-Sepharose and Superose 12. The molecular mass of the xylanase was estimated to be 23kDa, indicating that the enzyme consists of a catalytic domain only. The enzyme displayed an optimum pH of 6,a temperature optimum of 60℃, a pH stability range from 2 to 11 and thermal stability up to 40℃. The N-terminal amino acid sequence of GXYN was A-T-V-I-T-T-N-Q-T-G-T-N-N-G-I-Y-Y-S-F-W-, and sharing a high degree of similarity with the N-terminal sequence of xylanases B and C from Streptomyces lividans, indicating GXYN belongs to family G/11 of glycoside hydrolases. GXYN was inferior to xylanase B from Streptomyces lividans in the hydrolysis of insoluble xylan because of its lack of a xylan binding domain.
- 社団法人日本農芸化学会の論文
- 2000-02-23
著者
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Kobayashi H
National Food Research Institute
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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KANEKO Satoshi
Food Biotechnology Division, National Food Research Institute
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Kaneko Satoshi
Food Biotechnology Div. National Food Res. Inst.
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Kuno Atsushi
Research Center For Medical Glycoscience National Institute Of Advanced Industrial Science And Techn
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Kuno Atsushi
Department Of Obstetrics And Gynecology Suzuki Hospital
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KANEKO Satoshi
National Food Research Institute
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Kobayashi H
National Food Res. Inst. Ibaraki Jpn
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Kuno Atsushi
Department Of 2nd Internal Medicine Sapporo Medical University School Of Medicine
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Kiuchi Hiroshi
Research Development Section Kibun Food Chemifa Co. Ltd.
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Hayashi Kiyoshi
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Hayashi K
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Muramatsu Mizuho
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science
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Kaneko Satoshi
National Food Res. Inst.
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Kaneko S
Food Biotechnology Division National Food Research Institute
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IWAMATSU Shinnosuke
Department of Material and Biological Chemistry, Faculty of Science, Yamagata University
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Kaneko Shigenobu
National Institute Of Crop Science (nics)
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Iwamatsu Shinnosuke
Department Of Material And Biological Chemistry Faculty Of Science Yamagata University
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Hayashi K
Department Of Biochemical Sciences Kagoshima University
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Kusakabe I
Univ. Tsukuba Tsukuba Jpn
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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Muramatsu Mizuho
Institute Of Applied Biochemistry University Of Tsukuba
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Muramatsu Mizuho
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Hayashi Kiyoshi
National Food Research Institute
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