Preparation of Dye-labeled Alginate for the Assay of Alginate Lyase
スポンサーリンク
概要
- 論文の詳細を見る
Dabsyl-alginate, the dye-labeled substrate of alginate lyase, was prepared. Low-viscosity alginate, which was prepared by enzymatic degradation of sodium alginate, and tetramethylenediamine were conjugated by reductive amination. Then, dabsyl-Cl was coupled with the primary amino group of the aminobutyl-alginate. The assay for endo-alginate lyase using dabsyl-alginate was simple to use. The amount of dyed fragment released from the substrate increased with the amount of the enzyme.
- 社団法人日本農芸化学会の論文
- 1997-02-23
著者
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Murakami K
Research And Development Department Kibun Food Chemifa Co. Ltd.
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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YOSHIDA Shigeki
Institute of Applied Biochemistry, University of Tsukuba
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MURATA Katsumi
Research Development Section, Kibun Food Chemifa Co., Ltd.
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TAKEUCHI Toshio
Research and Development Department, Kibun Food Chemifa Co., Ltd.
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WATANABE Sayaka
Institute of Applied Biochemistry, University of Tsukuba
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Yoshida S
Plant Science Center Riken Institute
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Murata K
Aoba Kasei Co. Ltd.
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Yoshida S
Cancer Immunotherapy Center Nagoya Kyoritu Hospital
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Yoshida S
Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer
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Murakami K
Univ. Tsukuba Ibaraki Jpn
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Murata K
Kobe Oji Zoo Kobe Jpn
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Takeuchi Toshio
Research And Development Department Kibun Food Chemifa Co. Ltd.
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Yoshida Shigeki
Inst. Applied Biochem. Tsukuba Univ.
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Kusakabe I
Univ. Tsukuba Tsukuba Jpn
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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Watanabe Sayaka
Institute Of Applied Biochemistry University Of Tsukuba
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Yoshida Shigeki
Institute Of Applied Biochemistry University Of Tsukuba
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MURATA Katsumi
Research and Development Department, Kibun Food Chemifa Co., Ltd.
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