Purification and Characterization of α-Glucuronidase from Snail Acetone Powder
スポンサーリンク
概要
- 論文の詳細を見る
We have purified the p-nitrophenyl α-D-glucuronide-hydrolyzing enzyme (PNP-GAase) from snail (Helix pomatia) acetone powder by chromatographies on DEAE-cellulose, CM-Toyopearl, and Toyopearl HW-55F. The molecular weight of the enzyme was 180,000 by gel filtration chromatography with Superose 6, and 97, 000 by SDS-polyacrylamide gel electrophoresis, and the pI was 6.8 by isoelectric focusing. The enzyme showed the maximum activity at pH 3.0 and 50℃, and was stable at pH between 3.0-7.0 and up to 50℃. The enzyme activity was greatly inhibited by Hg^<2+>, p-chloromercuribenzoic acid, sodium dodecylsulfate, and N-bromosuccinimide. The enzyme released D-glucuronic acid not only from PNP-GA but also from 2-, 3-, and 4-O-α-D-glucuronosyl-D-xyloses, O-α-D-glucuronosyl α-D-glucuronide, and benzyl 4-O-α-D-glucuronosyl-β-D-glucoside. The results suggest that the α-glucuronidase from snail acetone powder had a broad substrate specificity comparing with α-glucuronidases from Aspergillus niger and basidiomycetes.
- 社団法人日本農芸化学会の論文
- 1995-06-23
著者
-
KOBAYASHI Hideyuki
National Astronomical Observatory of Japan
-
Kobayashi H
National Food Research Institute
-
Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
-
YOSHIDA Shigeki
Institute of Applied Biochemistry, University of Tsukuba
-
Kawabata Yasuyuki
Institute of Applied Biochemistry, University of Tsukuba
-
Yoshida S
Plant Science Center Riken Institute
-
Yoshida S
Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer
-
Kiuchi Hiroshi
Research Development Section Kibun Food Chemifa Co. Ltd.
-
Ono Kimihiro
Institute of Applied Biochemistry, University of Tsukuba
-
Gama Yasuo
Materials and Chemical Research Institute
-
Yoshida Shigeki
Inst. Applied Biochem. Tsukuba Univ.
-
Gama Y
National Inst. Advanced Industrial Sci. And Technol. (aist) Ibaraki Jpn
-
Kusakabe I
Univ. Tsukuba Tsukuba Jpn
-
Koyama Y
National Inst. Biosci. & Human Technol. Tsukuba Science City Jpn
-
Ono Kimihiro
Institute Of Applied Biochemistry University Of Tsukuba
-
Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
-
Kawabata Y
Univ. Tsukuba Ibaraki Jpn
-
Kawabata Yasuyuki
Institute Of Applied Biochemistry University Of Tsukuba
-
Yoshida Shigeki
Institute Of Applied Biochemistry University Of Tsukuba
関連論文
- Milliarcsecond-Scale Structure in the Gamma-Ray Loud Quasar PKS 1622-297
- VSOP Polarization Observing at 1.6 GHz and 5 GHz
- Screening for Milk-clotting Enzyme from Basidiomycetes
- Deodorization of Soybean Protein with Microorganisms
- Chemical Structure of Xylan in Cotton-seed Cake(Biological Chemistry)
- Preparation of Glucuronoxylo-oligosaccharides from an Acid Hydrolysate of Corn-hulls(Biological Chemistry)
- Action of Soymilk-clotting Enzyme from Bacillus sp. K-295G-7 on the Acidic Subunit of Soybean 11S Globulin(Biological Chemistry)
- Application of Soymilk-whey to Production of Beverage Containing α-D-Galactosyl Oligosaccharides(Food & Nutrition)
- Cloning and Sequence Analysis of cDNA for Irpex lacteus Aspartic Proteinase(Biological Chemistry)
- Substrate Specificity of Soymilk-clotting Enzymes I and II from Bacillus sp. K-295G-7 toward Peptide Substrates (Biological Chemistry)
- Coagulation of Proteins in Leguminous Milk by Commercial Proteinases(Food & Nutrition)
- Fiber-structured Cheese Making with Irpex lacteus Milk-clotting Enzyme(Food & Nutrition)
- Functional Properties of Three Soymilk Curds Prepared with an Enzyme, Calcium Salt and Acid(Food & Nutrition)
- Selection of Commercial Enzymes Suitable for Making Soymilk-curd(Food & Nutrition)
- Studies of the Coagulation of Soymilk-protein by Commercial Proteinases(Food & Nutrition)
- Purification and Some Properties of β-Mannanase from Penicillium purpurogenum
- Purification and Characterization of Soymilk-clotting Enzymes from Bacillus sp. K-295G-7
- Substrate Specificity of the Milk-clotting Enzyme from Irpex lacteus on Peptide Hormones
- Production and Properties of a Soymilk-clotting Enzyme System from a Microorganism
- Substrate Specificity of the Milk-clotting Enzyme from Irpex lacteus on κ- and β-Casein
- Substrate Specificity of the Milk-clotting Enzyme from Irpex lacteus on αs1-Casein
- Milk-clotting Enzyme from Irpex lacteus as a Calf Rennet Substitute for Cheddar Cheese Manufacture
- Purification and Characterization of Extracellular Poly(β-D-1, 4-mannuronide) Lyase from Dendryphiella salina IFO 32139
- Preparation of Dye-labeled Alginate for the Assay of Alginate Lyase
- Characterization of a Novel Alginate Lyase from Flavobacterium multivolum K-11
- Purification and Characterization of Endo Poly (α-L-Guluronate) Lyase in the Enzyme System from Flavobacterium multivolum
- Preparation of Two Series of Oligo-guluronic Acids from Sodium Alginate by Acid Hydrolysis and Enzymatic Degradation
- A Simple Method for Preparation of Poly-mannuronate Using Poly-guluronate Lyase
- Production of Xylose from Xylan with Intracellular Enzyme System of Aspergillus niger 5-16
- Structure of Rice-straw Arabinoglucuronoxylan and Specificity of Streptomyces Xylanase toward the Xylan(Biological Chemistry)
- H_2O Maser Outflow from the Red Supergiant Star NML Cygni Observed with Japanese VLBI Network
- The VLBI Space Observatory Programme and the Radio-Astronomical Satellite HALCA
- First mm-VLBI Observations between the TRAO 14-m and the NRO 45-m Telescopes : Observations of 86GHz SiO Masers in VY Canis Majoris
- The VSOP 5 GHz AGN Survey I. Compilation and Observations
- Specificity of β-Mannanase from Penicillium purpurogenum for Konjac Glucomannan(Biological Chemistry)
- Crystallization and Preliminary X-ray Crystallographic Study of Streptomyces olivaceoviridis E-86 β-Xylanase
- Kinetic Studies on p-Nitrophenyl-cellobioside Hydrolyzing Xylanase from Cellvibrio gilvus
- Preparation of β-1, 4-Mannobiose from White Copra Meal by a Mannanase from Penicillium purpurogenum
- An Easy Method for the Preparation of Sophorose from Stevioside
- Screening for Phenoloxidases from Edible Mushrooms(Biochemistry & Molecular Biology)
- Purification and Characterization of an α-L-Arabinofuranosidase from suspension cultured Non-embryo genic carrot (Daucus carota. L)
- PCR Cloning and Expression of the F/10 Family Xylanase Gene from Streptomyces olivaceoviridis E-86
- Substrate Specificity of the α-L-Arabinofuranosidase from Trichoderma reesei
- Substrate Specificites of α-L-Arabinofuranosidases Produced by Two Species of Aspergillus niger
- Purification and Characterization of α-Glucuronidase from Snail Acetone Powder
- Large Angle Bending of the Light-Month Jet in Centaurus A
- Asymmetric Free-Free Absorption towards a Double Lobe of OQ 208
- Kinetic Analysis of Temperature-Programmed Autoxidation of Ethyl Eicosapentaenoate and Ethyl Docosahexaenoate
- Kinetic Analysis of the Autoxidation of Ethyl Eicosapentaenoate at Different Oxygen Levels
- Primary Structure and Properties of Ribonuclease Bm2 (RNase Bm2) from Bryopsis maxima(Biochemistry)
- Amino Acid Sequence and Characterization of a Nuclease (Nuclease Le3) from Lentinus edodes(Biochemistry & Molecular Biology)
- Amino Acid Sequence of a Trypsin Inhibitor from a Spirometra(Spirometra erinaceieuropaei)
- Relationship to Two Ribonucleases with Molecular Masses of 45 kDa and 37 kDa from the Culture Medium of Lentinus edodes
- Amino Acid Sequence of a Nuclease (Nuclease Le1) from Lentinus edodes
- Amino Acid Sequence of an Unique Ribonuclease with a C-Terminus rich in O-Glycosylated Serine and Threonine from Culture Medium of Lentinus edodes
- Comparison of Base Specificity and Other Enzymatic Properties of Two Protozoan Ribonucleases from Physarum polycephalum and Dictyostelium discoideum
- Characterization and Primary Structure of a Base Non-Specific and Acid Ribonuclease from Dictyostelium discoideum
- Characterization of Ribonucleases from Culture Medium of Lentinus edodes
- Primary Structure of Base Non-specific and Acid Ribonuclease from Bullfrog (Rana catesbeiana)
- The Base Specificities of Tomato Ribonuclease (RNase LE) and Its Asp44 Mutant Enzyme Expressed from Yeast Cells
- Two-Epoch Space VLBI Observations of the Gamma-Ray Loud Quasar PKS 1741-038
- Syntheses of 4-Methylumbelliferyl-β-D-Xylobioside and 5-Bromo-3-Indolyl-β-D-Xylobioside for Sensitive Detection of Xylanase Activity on Agar Plates
- Purification and Characterization of a Family G/11 β-Xylanase from Streptomyces olivaceoviridis E-86
- Characterization of a Thermostable Endo-β-1,4-D-galactanase from the Hyperthermophile Thermotoga maritima
- Synergy Between an α-L-Arabinofuranosidase from Aspergillus oryzae and an Endo-Arabinanase from Streptomyces coelicolor for Degradation of Arabinan
- Expression of Streptomyces coelicolor α-Galactosidase Gene in Escherichia coli and Characterization
- Oxidation of Some Alkanes and Trichloroethylene by H_2O_2/Hydroxylase System of Soluble Methane Monooxygenase from Methylocystis sp.M
- Purification and Characterization of Component B of a Soluble Methane Monooxygenase from Methylocystis sp. M
- Purification, Characterization, and cDNA Cloning of a Novel α-Galactosidase from Mortierella vinacea
- Purification and Some Properties of α-Galactosidase from Penicillium purpurogenum
- Purification and Characterization of Recombinant Mortierella vinacea α-Galactosidases I and II Expressed in Saccharomyces cerevisiae
- Detection of α-L-Arabinofuranosidase Activity in Isoelectric Focused Gels using 6-Bromo-2-naphthyl-α-L-arabinofuranoside
- VLBI Monitoring of 3C 84 (NGC 1275) in Early Phase of the 2005 Outburst
- Galactose Depletion by Mortierella vinacea α-Galactosidase II Increases the Synergistic Interaction between Guar Gum and Xanthan Gum
- Nucleotide Sequence of α-Galactosidase cDNA from Mortierella vinacea
- In Vitro Sulfation of Glycoprotein with Sulfotransferase from Dictyosterium discoideum
- Characterization of Aspartic Proteinase from Basidiomycete, Laetiporus sulphureus
- Substrate Specificity of α-Glucuronidase Isolated from Snail Acetone Powder
- Effect of Replacing the Aspartic Acid / Glutamic Acid Residues of Bullfrog Sialic Acid Binding Lectin with Asparagine / Glutamine and Arginine on the Inhibition of Cell Proliferation in Murine Leukemia P388 Cells
- Structure of xylan from culms of bamboo grass (Sasa senanensis Rehd.)
- Effects of Unsaturated Uronic Acid Residues at Non-reducing End on Bond Cleavage Frequency of Poly(1, 4-α-L-guluronide) Lyase from Enterobacter cloacae M-1
- Substrate Specificities of α-Galactosidases from Yeasts
- A New Type of RNase T2 Ribonuclease in Two Basidiomycetes Fungi, Lentinus edodes and Irpex lacteus(Biochemistry & Molecular Biology)
- Stereoselective Synthesis of Regioisomers of Aldobiouronic Acid
- RECONSTITUTION OF TONOPLAST H^+-PPASE FROM RICE CULTURED CELLS INTO LIPOSOMES
- Purification and Characterization of Extracellular β-Galactosidase Secreted by Supension Cultured Rice (Oryza sativa L.) Cells(Biochemistry & Molecular Biology)
- Transglucosylation into Stevioside by the Enzyme System from Streptomyces sp.(Biological Chemistry)
- Autoproteolytic Processing of Aspartic Proteinase from Sunflower Seeds
- Purification and Characterization of Aspartic Proteinase from Sunflower Seeds
- PURIFICATION OF THE ASPARTIC PROTEINASE FROM SUNFLOWER SEEDS
- Enzymatic Preparation of Crystalline Glucose from Waste Cellulosic Materails
- HALCA' s Onboard VLBI Observing System
- Purification and Characterization of Intracellular α-Glucuronidase from Aspergillus niger 5-16
- Expression of Rice (Oryza sativa L. var. Nipponbare) α-Galactosidase Genes in Escherichia coli and Characterization
- Structure of Hardwood Xylan and Specificity of Streptomyces β-Xylanase toward the Xylan
- STRUCTURE OF THE VACUOLAR H^+ -PYROPHOSPHATASE GENES OF RICE (Oryza sativa L. )
- Release, Regeneration and Mutant Induction of Pleurotus cornucopiae ('Tamogitake') Protoplasts(Microbilolgy & Fermentation Industry)
- IMPACT EVALUATION OF SEA LEVEL RISE ON INDONESIAN COASTAL CITIES : MICRO APPROACH THROUGH FIELD SURVEY AND MACRO APPROACH THROUGH SATELLITE IMAGE ANALYSIS
- Design and Development of a High-Speed Data-Acquisition System for the Korean VLBI Network
- Crystallization and Preliminary X-Ray Crystallographic Study of Streptomyces olivaceoviridis E-86 .BETA.-Xylanase.