Primary Structure and Properties of Ribonuclease Bm2 (RNase Bm2) from Bryopsis maxima(Biochemistry)
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概要
- 論文の詳細を見る
A base non-specific ribonuclease (RNase Bm2) was isolated from a green algae (Ulvophyceae, Bryopsis maxima) as a single band on SDS-PAGE, and its primary structure and enzymatic properties, including base specificity, were investigated. The amino acid sequence of RNase Bm2 was homologous to many RNase T2 family RNases, and their characteristic CAS sequences were also conserved. The molecular mass of RNase Bm2 was 24444 Da, and its optimal pH was 5.5. RNase Bm2 was a poly U preferential RNase, similar to RNase MC1 from bitter gourd. The base specificity of this RNase suggested that the base specificity of the B1- and B2-base binding sites of RNase Bm2 were G≧U>C≫A and U>G>C≫A, respectively. The estimated active site of RNase Bm2 was very similar to that of RNase MC1 from bitter gourds; however, a tyrosine residue at the B1-base binding site that is conserved for all RNase T2 family RNases was replaced by a tryptophan residue. Here we discuss the effect of this replacement on the base specificity of RNase Bm2 and the phylogenetic relationship of RNase T2 family enzymes.
- 社団法人日本薬学会の論文
- 2006-05-01
著者
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Koyama Takashi
Department of Cardiovascular and Respiratory Medicine, Akita University Graduate School of Medicine
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Kobayashi H
National Food Research Institute
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Koyama Hideki
Department of Oral Pathology, Health Sciences University of Hokkaido
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Koyama Takashi
Division Of Life Sciences Graduate School Of Natural Science And Technology Kanazawa University
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Kobayashi H
National Food Res. Inst. Ibaraki Jpn
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Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
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Ohgi Kazuko
Department of Microbiology, Hoshi College of Pharmacy
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Ohgi K
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi Kazuko
Department Of Microbiology Hoshi College Of Pharmacy
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Irie M
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Irie Masachika
Department Of Microbiology Hoshi College Of Pharmacy
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Kiuchi Hiroshi
Research Development Section Kibun Food Chemifa Co. Ltd.
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Hayashi K
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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KOBAYASHI Hiroko
Department of Internal Medicine II, Fukushima Medical University School of Medicine
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Koyama Hideki
Deparment Of Oral Pathology School Of Dentistry Health Sciences University Of Hokkaido
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Koyama Takashi
Department Of Microbiology College Of Pharmacy Nihon University
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ITAGAKI Tadashi
Department of Microbiology, College of Pharmacy, Nihon University
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DAIGO Satoshi
Department of Microbiology, College of Pharmacy, Nihon University
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IWAMA Masanori
Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
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INOKUCHI Norio
Department of Microbiology, College of Pharmacy, Nihon University
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Daigo Satoshi
Department Of Microbiology College Of Pharmacy Nihon University
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Koyama Hideki
Department Of Microbiology College Of Pharmacy Nihon University
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Itagaki Tadashi
Department Of Microbiology College Of Pharmacy Nihon University
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Irie M
Hoshi Coll. Pharmacy Tokyo Jpn
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Iwama M
Department Of Microbiology Faculty Of Pharmaceutical Sciences Ohu-university
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Inokuchi N
Department Of Microbiology College Of Pharmacy Nihon University
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Kobayashi Hiroko
Department Of Gastroenterology And Rheumatology Fukushima Medical University School Of Medicine
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Ohgi Kazuko
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi K
Department Of Microbiology Hoshi College Of Pharmacy
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Koyama Takashi
Department of Cardiology, Akita University School of Medicine
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Kobayashi Hiroko
Department of Cardiovascular Disease Research, Eisai Tsukuba Research Laboratories
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